AIB Antibody
Description
AIB System for Pathogen Detection
The AIB (Antibody-Immuno-NHS-Bead) system is a rapid diagnostic platform that combines monoclonal antibodies (mAbs) with functionalized NHS beads for visual detection of pathogens like Salmonella. Developed to address limitations in traditional methods, this system operates without specialized equipment .
Key Components:
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NHS bead matrix: Provides covalent binding sites for antibody conjugation
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HRP-conjugated mAbs: Paired antibodies (2F1 and 1B12) exhibit high affinity (K<sub>D</sub> = 3.677 nM and 1.126 nM, respectively)
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Colorimetric substrate: Produces visible blue coloration upon pathogen detection
Performance Metrics:
| Parameter | AIB System | PCR | LAMP |
|---|---|---|---|
| Sensitivity (CFU/mL) | 7–9 ×10⁰ | 10² | 10¹ |
| Detection Time | <1 hour | 3–4 hours | 2–3 hours |
| Equipment Required | None | Thermal cycler | Real-time PCR |
| Sample Types Tested | Milk, eggs | Milk, eggs | Milk, eggs |
The AIB system demonstrated 99.45% linear correlation (R² = 0.9945) between optical density and Salmonella concentration, outperforming PCR and LAMP in sensitivity for artificially contaminated samples .
Antibodies Targeting AIB-Modified Antigens
AIB (α-aminoisobutyric acid), a non-proteinogenic amino acid, enhances antigenicity when incorporated into synthetic peptides. Studies on Plasmodium falciparum LSA-1 antigens showed:
While not directly related to AIB antibodies, AI-driven methods like IgFold (antibody structure prediction) and IsAb2.0 (computational design) highlight innovations in antibody engineering:
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IgFold: Predicts antibody structures in <25 seconds with 0.43–0.53 Å RMSD accuracy for framework residues .
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IsAb2.0: Optimizes antibody-antigen binding affinity using AlphaFold-Multimer and FlexddG, achieving 10-fold affinity improvements in HIV-neutralizing nanobodies .
Limitations and Future Directions
Product Specs
Target Background
Function and Gene References:
- Negative Regulation of Jasmonic Acid Response: Studies suggest that JAM1, JAM2, and JAM3 redundantly act as negative regulators of jasmonic acid-mediated male fertility (PMID: 24056034). JAM1 (At2g46510) specifically negatively regulates the JA response, largely antagonistically to MYC2 (PMID: 23852442). Competitive binding between JAM1 and MYC2 to MYC2 target sequences likely underlies this negative regulation of jasmonate signaling and suppression of MYC2 function (PMID: 23673982).
- Other Gene Expression Effects: Some genes with WRKY or bHLH cis-elements in their promoter regions exhibit elevated transcript levels compared to wild-type (PMID: 22948308).
- ABA Signaling: AtAIB functions as a transcription activator in the regulation of ABA signaling in Arabidopsis (PMID: 17828375).
