AIFM2 Antibody

Introduction to AIFM2 Antibody

The AIFM2 Antibody is a highly specific immunological reagent designed to detect the Apoptosis-Inducing Factor, Mitochondrion-Associated 2 (AIFM2) protein, also known as Ferroptosis Suppressor Protein 1 (FSP1). AIFM2 is a flavoprotein oxidoreductase involved in mitochondrial thermogenesis, lipid metabolism, and ferroptosis suppression . The antibody is widely used in molecular biology research to study AIFM2 localization, expression levels, and functional roles in diseases such as cancer and metabolic disorders .

3.1. Thermogenesis and Lipid Metabolism

AIFM2 localizes to lipid droplets (LDs) and mitochondria in brown adipose tissue (BAT), where it sustains glycolysis and thermogenesis by oxidizing NADH to NAD . Immunoblotting with AIFM2 Antibody revealed its presence in LD fractions after sucrose gradient centrifugation, confirming its role in lipid-associated pathways .

3.2. Ferroptosis Suppression

AIFM2 reduces oxidized coenzyme Q10 (ubiquinone) to ubiquinol, mitigating lipid peroxidation and ferroptosis . Studies using AIFM2 Antibody (20886-1-AP) demonstrated that AIFM2 knockdown increases ferroptosis sensitivity in cancer cells, while overexpression enhances antioxidant defense .

3.3. Cancer Metastasis

AIFM2 promotes hepatocellular carcinoma (HCC) metastasis by activating mitochondrial biogenesis via SIRT1/PGC-1α signaling . Western blotting with AIFM2 Antibody confirmed its upregulation in HCC tissues, correlating with poor patient outcomes .

Published Applications and Protocols

Protocols for AIFM2 Antibody use include optimized dilutions (e.g., 1:5000 for WB) and antigen retrieval methods (TE buffer pH 9.0 for IHC) .

Clinical and Diagnostic Potential

AIFM2 antibodies are critical for diagnosing AIFM2-related pathologies, including obesity and cancer. Their application in CoIP assays has revealed novel interactions with mitochondrial proteins like GPX4, a key ferroptosis regulator .