ankib1 Antibody

ANTIBODY CHARACTERIZATION

Target Protein: ANKIB1 (UniProt ID: Q9P2G1) is a 122 kDa protein containing ankyrin repeats, RING-IBR-RING (RBR) domains, and a variant Ariadne domain . It functions as an E3 ubiquitin ligase or a component of E3 complexes, facilitating substrate ubiquitination .

Functional Insights

• E3 Ubiquitin Ligase Activity: ANKIB1 interacts with E2 enzymes (UbcH8, UbcH10, UbcH13) to mediate K48-linked polyubiquitination, promoting proteasomal degradation of substrates .

• Subcellular Localization: Predominantly cytoplasmic (122 kDa isoform), with a minor nuclear 88 kDa isoform .

• Tissue-Specific Expression:

  • Brain: Highly expressed in the cortex but minimal in basal ganglia .

  • Cancer: Differential expression observed in immortalized lymphocytes and tumor cell lines .

Pathological Relevance

• Neurodegeneration: Linked to cerebral cavernous malformations and neurodegenerative disorders .

• Immune Regulation: ANKIB1 suppresses retinoic acid-inducible gene-I (RIG-I)-like receptor (RLR) signaling by degrading mitochondrial antiviral signaling protein (MAVS), thereby attenuating antiviral interferon responses .

Key Assays

• Western Blot: Detects endogenous ANKIB1 at 122 kDa in HEK293T, HeLa, and A431 cells .

• Immunohistochemistry: Strong cortical staining in rat brain; variable expression in mouse kidney and placenta .

• Immunoprecipitation: Confirmed interaction with MAVS and E2 enzymes .

Technical Performance

• Dilution Guidelines:

  • WB: 1:2000

  • IHC/IF: 1:200

• Cross-Reactivity: No off-target binding reported in human or mouse models .

APPLICATIONS IN RESEARCH

• Ubiquitination Studies: Used to identify E3 ligase substrates and mechanisms .

• Neurological Research: Investigates ANKIB1’s role in brain development and disease .

• Cancer Biology: Explores differential expression in tumorigenesis .