FBA3 Antibody
Description
Introduction to FBA3 Antibody
The FBA3 Antibody (PHY7224S) is designed to detect and study the FBA3 enzyme, which catalyzes the cleavage of fructose-1,6-bisphosphate in the Calvin cycle and glycolysis. It is widely used in plant biology research to investigate photosynthesis, carbohydrate metabolism, and stress responses .
Cross-Reactivity and Specificity
The antibody exhibits broad cross-reactivity due to sequence conservation among plant FBA isoforms:
| Species/Protein | Homology to FBA3 Immunogen | Cross-Reactivity Confirmed |
|---|---|---|
| FBA1 (AT2G21330) | 90% (18/20 residues) | Yes |
| FBA2 (AT4G38970) | 80% (16/20 residues) | Yes |
| Spinacia oleracea | Not specified | Yes (via Western blot) |
| Vitis vinifera | Not specified | Yes |
This cross-reactivity enables comparative studies of aldolase isoforms across plant species .
Research Applications
Validated for use in:
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Western blotting: Detects FBA3 in lysates from Arabidopsis, rice, and soybean.
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Immunohistochemistry (IHC): Localizes FBA3 in chloroplasts of plant tissues.
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ELISA: Quantifies FBA3 expression under stress conditions (e.g., drought, salinity) .
Key Considerations
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Specificity Limitations: Cross-reactivity with FBA1/FBA2 requires careful experimental controls.
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Storage Stability: Lyophilized format ensures long-term stability but mandates strict adherence to storage protocols .
Research Implications
While direct studies using this antibody are not detailed in public literature, its design supports investigations into:
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Metabolic adaptation in crops under climate stress.
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Evolutionary conservation of aldolase function in plants.
Product Specs
Composition: 50% Glycerol, 0.01M PBS, pH 7.4
