Apoa1 Antibody, Biotin conjugated
Description
Definition and Mechanism
ApoA1 is the primary protein of HDL, playing a central role in cholesterol efflux and cardiovascular health . The biotin-conjugated antibody enables immunoassay techniques such as ELISA or Western blot by binding to ApoA1 via its biotin tag, which facilitates detection through avidin-based systems .
Applications
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ELISA: Used as detection antibodies in sandwich assays. For example, the mouse monoclonal mHDL36 (rat host) pairs with mHDL93 for mouse ApoA1 detection .
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Western Blot: Detects ApoA1 in denaturing gels. The rabbit polyclonal CSB-PA001913LD01RA recommends dilutions of 1:1000–1:5000 .
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Imaging Techniques: Polyclonal antibodies like LAA519Hu71 facilitate immunocytochemistry and immunohistochemistry .
Role in Lipid Metabolism
ApoA1 interacts with SIDT2 to enhance cholesterol efflux via HDL particles. Overexpression of SIDT2 mutants disrupts this interaction, reducing ApoA1 secretion by 37% in hepatocytes .
Bone Marrow Adipocytes
ApoA1 deficiency in mice correlates with reduced hematopoietic stem cells and beige adipocyte markers (e.g., osteopontin, VEGF), highlighting its role in bone marrow microenvironment regulation .
Cross-Reactivity and Specificity
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- The pharmacokinetics and pharmacodynamics of apoA-I peptide are significantly influenced by both the route of administration and the formulation. PMID: 27881716
- N-acetylcysteine, by reducing ABCA1 and increasing ABCG1, counteracts the decrease in apo A-I-mediated cholesterol efflux. PMID: 25305669
- Apolipoprotein A-I induces tubulin phosphorylation in conjunction with cholesterol release in fetal rat astrocytes. PMID: 24814947
- ApoA-I may serve as a protective blood-borne factor involved in the remote ischemic preconditioning mechanism. PMID: 24155931
- It plays a critical role in maintaining cholesterol homeostasis. PMID: 24117066
- In neonatal rat ventricular cardiomyocytes, the aldosterone-dependent chronotropic effects of antiapoA-1 IgG involve the nongenomic activation of L-type calcium channels. PMID: 22253414
- The translocation of cholesterol and phospholipid into the cytosol is related to the apo A-I-mediated HDL assembly in astrocytes through functional association with caveolin-1 and a cyclosporin A-sensitive cyclophilin protein(s). PMID: 11773045
- ABCA1-dependent cholesterol mobilization to apoA-I increased new cholesterol synthesis, indicating depletion of the regulatory pool of hepatocyte cholesterol during HDL formation. PMID: 14993246
- The association of CLPP with microtubules is mediated by caveolin-1, induced by apoA-I and regulates intracellular cholesterol trafficking for assembly of cellular lipids to apoA-I-HDL. PMID: 16606364
- Data demonstrate that a complex of apolipoprotein A-I and pregnenolone modulates the rate of protein biosynthesis in liver cells. PMID: 18457017
- ApoA-I reduces I/R-induced inflammatory responses, decreases renal microscopic damage, and improves renal function. ApoA-I protects the kidney from I/R injury by inhibiting inflammatory cytokine release and neutrophil infiltration and activation. PMID: 18535924
