APRR7 Antibody

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Description

Definition and Biological Context

PRR7 is a member of the Pseudo-Response Regulator family, which includes PRR9, PRR5, PRR3, and TOC1. These proteins form a transcriptional-translational feedback loop that regulates circadian rhythms and photoperiodic flowering . The PRR7 antibody specifically targets this protein, enabling its detection, quantification, and functional analysis in plant tissues.

Table 1: Phenotypic Effects of PRR7 Mutations

GenotypeCO Protein LevelsFT mRNA LevelsFlowering Time
Wild-TypeHighHighNormal (Early)
prr7 prr9 mutantReduced (~30% of WT)Drastically ReducedDelayed by 10–14 days

Validation and Specificity

Antibody validation follows stringent criteria :

  • Specificity: Verified using prr7 knockout lines, showing no cross-reactivity with other PRR family members .

  • Reproducibility: Consistent results across diurnal time courses and independent labs .

  • Functional Assays: Rescue of CO stabilization in PRR7-overexpressing lines confirms antibody efficacy .

Future Directions

Current research gaps include:

  • Structural Analysis: Mapping PRR7 epitopes to refine antibody specificity.

  • Cross-Species Relevance: Testing reactivity in crops (e.g., rice, wheat) for agricultural applications.

Product Specs

Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
APRR7 antibody; At5g02810 antibody; F9G14_120Two-component response regulator-like APRR7 antibody; Pseudo-response regulator 7 antibody
Target Names
APRR7
Uniprot No.

Target Background

Function
APRR7 functions as a transcriptional repressor of CCA1 and LHY, while acting as a positive regulator of LWD1 and LWD2 expression. This protein represses the expression of other clock proteins and key regulators of plant growth, development, and response to abiotic stress. APRR7 is involved in the intricate positive and negative feedback loops of the circadian clock. It plays a critical role in controlling photoperiodic flowering response and temperature compensation. The expression of several members of the ARR-like family is regulated by the circadian rhythm. Notably, APRR9, APRR7, and APRR5 work in coordination to repress the expression of target genes from noon until midnight. The specific coordinated sequential expression of APRR9, APRR7, APRR5, APRR3, and APPR1 generates circadian waves that are believed to underpin the endogenous circadian clock.
Gene References Into Functions
  1. Phytochrome B-mediated germination necessitates the presence of functional PSEUDO-RESPONSE REGULATOR 7 (PRR7) and TIMING OF CAB EXPRESSION 1 (TOC1) alleles. PMID: 28941290
  2. PRR7 is implicated in the oxidative stress response and the regulation of stomata conductance. PMID: 23808423
  3. PRR5 and PRR7 collaborate (or synergistically) to perform essential clock-associated functions in proximity to the central oscillator. PMID: 15695441
  4. Research findings demonstrate that overexpression of PRR7 leads to significantly compromised circadian rhythms. Moreover, the PRR7 protein undergoes phosphorylation in a circadian-regulated manner, and its levels are subject to post-translational regulation by both diurnal and circadian mechanisms. PMID: 17877705
Database Links

KEGG: ath:AT5G02810

STRING: 3702.AT5G02810.1

UniGene: At.20552

Protein Families
ARR-like family
Subcellular Location
Nucleus.

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