PRR7 is a member of the Pseudo-Response Regulator family, which includes PRR9, PRR5, PRR3, and TOC1. These proteins form a transcriptional-translational feedback loop that regulates circadian rhythms and photoperiodic flowering . The PRR7 antibody specifically targets this protein, enabling its detection, quantification, and functional analysis in plant tissues.
Genotype | CO Protein Levels | FT mRNA Levels | Flowering Time |
---|---|---|---|
Wild-Type | High | High | Normal (Early) |
prr7 prr9 mutant | Reduced (~30% of WT) | Drastically Reduced | Delayed by 10–14 days |
Antibody validation follows stringent criteria :
Specificity: Verified using prr7 knockout lines, showing no cross-reactivity with other PRR family members .
Reproducibility: Consistent results across diurnal time courses and independent labs .
Functional Assays: Rescue of CO stabilization in PRR7-overexpressing lines confirms antibody efficacy .
Current research gaps include:
Structural Analysis: Mapping PRR7 epitopes to refine antibody specificity.
Cross-Species Relevance: Testing reactivity in crops (e.g., rice, wheat) for agricultural applications.