ARI6 Antibody
Description
Overview of ARI6 Antibody
The ARI6 antibody refers to a polyclonal or monoclonal antibody targeting a protein or epitope associated with the Ari6 quantitative trait locus (QTL), a genetic region linked to antibody response regulation. This locus was identified in Collaborative Cross (CC) mouse strains as a key modulator of IgG2b antibody production against influenza A virus (IAV) at day 15 post-infection . ARI6 antibodies are primarily research tools used to study immune response mechanisms, genetic determinants of humoral immunity, and host-pathogen interactions.
Antibody Response Dynamics
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IgG2b Specificity: ARI6-linked antibodies exhibit a strong correlation with IgG2b production, an isotype critical for complement activation and Fcγ receptor-mediated phagocytosis .
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Temporal Regulation: The Ari6 locus influences antibody kinetics, peaking at 15 days post-IAV infection, unlike other Ari loci (e.g., Ari1–Ari5) that affect earlier response phases .
Strain-Specific Effects
NZO/HlLtJ mice carrying the high-response haplotype show 2.3-fold higher IgG2b titers compared to low-response strains (e.g., C57BL/6J) . This suggests genetic polymorphisms in Ari6 modulate B-cell activation or class-switching efficiency.
Functional Validation
In CC-F1 hybrid mice:
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Ari6 haplotypes directly correlated with viral neutralization capacity (r = 0.72, p < 0.01) .
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No association was observed with antibody responses to SARS-CoV or chikungunya virus, indicating pathogen specificity .
Comparative Analysis of Ari Loci
| Locus | Chromosome | Target Isotype | Pathogen Association |
|---|---|---|---|
| Ari1 | 17 | IgG2a/IgG2c | IAV-specific |
| Ari2 | 11 | IgG3 | Broad (IAV, SARS-CoV) |
| Ari6 | 7 | IgG2b | IAV-specific |
| Ari8 | 15 | Total IgG | Late-phase response (days 15–45) |
Implications for Biomedical Research
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Vaccine Development: Ari6 haplotypes may inform adjuvants targeting IgG2b-class switching for improved influenza vaccines.
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Personalized Immunology: Genetic screening for Ari6 analogs in humans could predict antibody response variability.
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Animal Model Optimization: NZO/HlLtJ mice serve as a high-responder model for studying IgG2b-dominant infections.
Limitations and Future Directions
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Mechanistic Uncertainty: The exact gene(s) underlying Ari6 remain unidentified, necessitating CRISPR-based validation studies.
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Human Translation: No direct orthologs of mouse Ari6 have been confirmed in human genome-wide association studies.
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
Q&A
Here’s a structured collection of FAQs tailored for academic researchers working with ARI6 antibodies, organized by complexity and methodological focus:
Advanced Research Questions
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Resolving contradictory data in ARI6 ubiquitin ligase activity assays
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Troubleshooting framework:
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Epitope mapping strategies for ARI6 antibody characterization
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Quantifying ARI6 dynamics during stress responses
