ARPC4 Antibody

Definition and Target

ARPC4 antibody specifically binds to the ARPC4 protein (20 kDa), a core subunit of the Arp2/3 complex. This complex mediates actin nucleation and branching, essential for cellular processes like motility, cytokinesis, and DNA repair .

Structure and Function of ARPC4

• Role in Actin Dynamics: The Arp2/3 complex, including ARPC4, promotes branched actin network formation, driving cell migration and membrane protrusions .

• Nuclear Functions: Regulates nuclear actin polymerization for DNA repair (e.g., homologous recombination) and transcriptional regulation .

• Pathological Relevance: Overexpressed in colorectal, bladder, and lung cancers, correlating with metastatic potential .

Applications of ARPC4 Antibody

Table 1: Functional Insights from ARPC4 Silencing Studies

Critical Considerations

• Validation: Always confirm antibody specificity using knockout controls or siRNA (e.g., reduced ARPC4 signal in silenced SW620 cells) .

• Storage: Stable at -20°C; avoid freeze-thaw cycles .

• Cross-Reactivity: Some antibodies (e.g., Proteintech’s 10930-1-AP) detect bovine homologs .

Disease Associations

• Cancer: ARPC4 overexpression linked to metastasis in colorectal, bladder, and lung cancers .

• Developmental Disorders: Mutations in ARPC4 cause developmental delay, language impairment, and ocular abnormalities (DEVLO syndrome) .

Future Directions

ARPC4 antibodies are pivotal for studying:

• Metastasis mechanisms via actin remodeling pathways.

• Nuclear actin’s role in genome stability and DNA repair .

• Host-pathogen interactions (e.g., M. tuberculosis evasion strategies) .