ASB8 Antibody

Key Functional Insights:

• Ubiquitin-Proteasome Pathway: ASB8 facilitates the degradation of specific proteins, including those involved in interferon signaling. For example, ASB8 regulates TBK1/IKKi homeostasis, modulating antiviral immune responses .

• Immune Modulation: Studies suggest ASB8 negatively regulates interferon pathways, highlighting its role in balancing immune activation .

A. Experimental Use Cases

• Western Blot: Detects ASB8 in human heart tissue and neuroblastoma cell lines (e.g., IMR32) .

• Immunohistochemistry: Identifies ASB8 in human colon carcinoma and rat skin tissues .

• Immunofluorescence: Localizes ASB8 in HepG2 and HeLa cells .

B. Key Findings from Studies

1. Ubiquitin Ligase Activity:
   ASB8 forms part of an ECS (Elongin-Cullin-SOCS-box) E3 ligase complex, critical for protein quality control .

2. Interferon Regulation:
   ASB8-mediated ubiquitination of TBK1/IKKi limits excessive interferon production, suggesting therapeutic potential in autoimmune diseases .

Clinical and Research Implications

• Cancer Research: ASB8 is detected in colon carcinoma tissues, hinting at roles in tumor biology .

• Autoimmune Disorders: Its regulatory effect on interferon pathways positions ASB8 as a potential drug target .

Limitations and Future Directions

• Species Specificity: Current antibodies are validated only for human, mouse, and rat samples .

• Mechanistic Gaps: The exact substrates of ASB8 beyond TBK1/IKKi remain unclear, necessitating further proteomic studies.