ASHR1 Antibody
Description
Definition and Biological Role of ASGR1 Antibodies
ASGR1 antibodies are immunological tools designed to detect or modulate the Asialoglycoprotein Receptor 1 (ASGR1), a C-type lectin expressed exclusively on hepatic parenchymal cells. This receptor facilitates the clearance of desialylated glycoproteins via endocytosis and lysosomal degradation . ASGR1 forms a hetero-oligomeric complex with ASGR2, with approximately 1 million copies per hepatocyte, making it a high-density target for therapeutic and diagnostic applications .
Research Use Cases
Therapeutic Development
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AMG 529: A monoclonal antibody tested in a Phase 1 trial (NCT03159425) showed dose-dependent increases in alkaline phosphatase (ALP), a biomarker of ASGR1 inhibition, with no severe adverse events .
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Catch-and-Release (CAR) Antibodies: Engineered to dissociate from ASGR1 in endosomes, CAR variants (e.g., CAR-WT) demonstrated 58–85x higher serum exposure than non-CAR antibodies in murine models .
Pharmacokinetic Profiles
| Antibody Type | Dose (mg/kg) | AUC (µg·h/mL) | Clearance Rate |
|---|---|---|---|
| Non-CAR-WT | 0.3 | 12.3 | Rapid (<24h) |
| CAR-WT | 0.3 | 1,045 | Sustained |
| AMG 529 (700 mg SC) | - | - | Target saturation observed |
Disease Associations
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SARS-CoV-2 Infection: ASGR1 facilitates viral entry into hepatocytes. Antibody blockade reduced pseudovirus infection in HepG2 cells by >60% .
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Liver Injury: ASGR1-deficient mice exhibited elevated ALT/AST, hepatic apoptosis, and fibrosis .
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Cardiometabolic Benefits: ASGR1 loss-of-function variants correlate with reduced coronary heart disease risk .
Challenges and Future Directions
Product Specs
Target Background
STRING: 3702.AT2G17900.1
Q&A
Given the lack of specific information about "ASHR1 Antibody" in the search results, I will create a general FAQ for researchers focusing on antibody-related research, emphasizing experimental design, data analysis, and advanced research questions. This approach will ensure that the content is both scientifically deep and relevant to academic research scenarios.
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To evaluate the specificity of an antibody, you should use a combination of techniques such as Western blotting, immunofluorescence, and ELISA. Include controls with known positive and negative samples to assess cross-reactivity. Use multiple cell lines or tissues to confirm specificity across different contexts.
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Use statistical methods like ANOVA or t-tests to compare binding efficiencies across different samples. For more complex datasets, consider using machine learning algorithms to identify patterns that might explain discrepancies. Always ensure that your sample size is sufficient and that data normalization procedures are applied to reduce systematic bias .
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To investigate the role of ASHR1 antibody in immune modulation, design experiments that involve blocking or activating the target antigen in cell cultures or animal models. Use flow cytometry to analyze changes in immune cell populations and cytokine profiling to assess functional outcomes.
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Optimizing antibody-based assays involves selecting the appropriate antibody concentration, incubation times, and blocking conditions to minimize background noise. Use serial dilutions to determine the optimal concentration for your specific application. Additionally, consider the use of secondary antibodies that enhance signal detection.
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Inconsistent results from different antibody batches can arise from variations in production, purification, or storage conditions. Perform side-by-side comparisons using the same experimental setup to identify batch-specific differences. Consider using alternative antibodies or validating results with independent methods like qPCR or sequencing.
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Yes, ASHR1 antibody can be used for single-cell analysis using techniques like single-cell Western blotting or imaging flow cytometry. These methods allow for the detection of protein expression at the single-cell level, providing insights into cellular heterogeneity.
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Conduct a thorough literature review to identify gaps in current knowledge and ensure your research question aligns with existing studies. Collaborate with experts in related fields to gain insights into the broader implications of your findings and to refine your research design .
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When using antibodies in animal studies, ensure that all procedures are approved by an ethics committee and adhere to guidelines for animal welfare. Minimize animal numbers and consider alternatives like in vitro models when possible.
Example Data Table: Antibody Optimization
| Antibody Concentration | Incubation Time | Signal-to-Noise Ratio |
|---|---|---|
| 1:100 | 1 hour | 3:1 |
| 1:500 | 1 hour | 5:1 |
| 1:100 | 2 hours | 4:1 |
| 1:500 | 2 hours | 6:1 |
This table illustrates how varying antibody concentration and incubation time can affect assay sensitivity.
Research Findings Example
In a recent study, researchers used ASHR1 antibody to investigate immune cell modulation. They found that blocking the ASHR1 antigen resulted in a significant decrease in inflammatory cytokine production, suggesting a potential therapeutic application for autoimmune diseases.
