ASIL1 Antibody

Biological Context of ASIL1

ASIL1 (Arabidopsis 6b-interacting protein 1-like 1) is a plant-specific trihelix transcription factor that represses embryonic and seed maturation genes during vegetative growth in Arabidopsis thaliana. It binds GT-box promoter elements (e.g., in the At2S3 gene) to suppress storage protein accumulation and lipid biosynthesis in non-seed tissues . ASIL1 also ensures proper timing of seed filling by regulating master regulators like LEC2, FUS3, and ABI3 during silique development . Structurally, ASIL1 contains:

• A trihelix DNA-binding domain

• Nuclear localization signals (SV40-like, MAT 2-like, bipartite-like)

• A Gly-rich motif and Pro-rich repressor domain

Antibody Development for Related Targets

While no commercial antibodies targeting ASIL1 are described in the provided sources, research on structurally similar proteins like ASXL1 (additional sex combs-like 1) offers technical parallels. ASXL1, a Polycomb group protein in humans, regulates chromatin remodeling and gene repression.

Key Antibodies for ASXL1

Validation Data

• Proteintech ASXL1 Antibody detects endogenous ASXL1 in HeLa, K-562, and MCF-7 cells via WB at 1:500–1:2000 dilution .

• Santa Cruz 6E2 Antibody confirms ASXL1’s nuclear localization and interaction with Polycomb repressive complexes in immunoprecipitation assays .

Functional Characterization (Parallel Example)

In studies on hASIC1a-blocking antibodies (e.g., ASC06-IgG1) :

• Binding Affinity: Surface plasmon resonance (SPR) and flow cytometry quantified ASC06-IgG1’s interaction with ΔhASIC1a (IC₅₀ = 85 nM).

• Functional Inhibition: Reduced acid-induced currents by 80% in CHO-K1 cells expressing hASIC1a .

Tissue Staining Protocols

For nuclear targets like ASXL1:

1. Epitope Retrieval: Heat-induced retrieval using Antigen Retrieval Reagent-Basic .

2. Detection: HRP-conjugated secondary antibodies with DAB chromogen .

Implications for Plant Biology Research

While ASIL1-specific antibodies are not commercially documented, lessons from ASXL1 studies highlight:

• Reproducibility: Target full-length proteins or conserved domains (e.g., trihelix motifs) for antibody design.

• Functional Assays: Combine ChIP-seq (to map GT-box binding) with mutant analyses (asil1-1) .

Research Gaps and Opportunities

1. Antibody Development: No ASIL1-specific antibodies are reported; plant-specific epitopes (e.g., Pro-rich domain) could guide future projects.

2. Cross-Reactivity: ASXL1 antibodies do not recognize ASIL1 due to divergent sequences .