ATHB-17 Antibody
Description
Molecular Function of ATHB-17
ATHB-17 is a class II HD-Zip transcription factor that acts as a transcriptional repressor containing an EAR-like motif, critical for modulating plant stress tolerance . It coordinates nuclear- and chloroplast-encoded photosynthetic genes (NEPGs and PEGs) under abiotic stress, enhancing stress resilience by:
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Repressing NEPGs (e.g., FDA6, LHCA2, LHB1B1, LHB1B2, PSBO1) via direct promoter binding .
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Activating PEGs (e.g., PSBA, PSBDC) indirectly through ATSIG5, a plastid sigma factor .
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Balancing photosystem stoichiometry to protect photosynthesis machinery .
Research Applications of ATHB-17 Antibody
The antibody is primarily used for:
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Chromatin Immunoprecipitation (ChIP): Validating ATHB-17’s direct binding to target gene promoters (e.g., ATSIG5) .
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Subcellular Localization: Confirming nuclear localization via GFP fusion assays .
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Protein Expression Analysis: Detecting HA-tagged ATHB-17 in overexpression lines .
Table 1: Key ATHB-17-Regulated Genes
| Gene Type | Example Genes | Regulatory Role | Binding Confirmed? |
|---|---|---|---|
| NEPGs | FDA6, LHCA2 | Repression via direct binding | Yes |
| PEGs | PSBA, PSBDC | Indirect activation | No |
| Regulators | ATSIG5 | Direct activation | Yes |
Table 2: Recombinant ATHB-17 Protein Variants
| Code | Source | Conjugate | Purity |
|---|---|---|---|
| CSB-YP854669DOA | Yeast | None | High |
| CSB-EP854669DOA | E. coli | None | High |
| CSB-BP854669DOA | Baculovirus | None | High |
Key Features:
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Epitope Tags: HA-tagged variants enable detection using anti-HA antibodies .
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Repression Domain: Truncated forms (e.g., ATHB17Δ113) lack the N-terminal repression domain, altering function .
Functional Insights from Genetic Studies
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Overexpression (OX) Lines: Show enhanced salt/drought tolerance, reduced oxidative damage, and increased photosynthetic capacity .
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Knockout (KO) Mutants: Exhibit heightened stress sensitivity and dysregulated PEG/NEPG expression .
Table 3: Stress Response Phenotypes
| Condition | ATHB17 OX | ATHB17 KO |
|---|---|---|
| Salt Stress | Increased survival | Reduced survival |
| Drought | Delayed wilting | Accelerated wilting |
| ROS Levels | Lower H2O2 | Higher H2O2 |
Mechanistic Model
Under stress, ATHB-17:
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Binds NEPG promoters (e.g., LHB1B1) to suppress light-harvesting genes .
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Activates ATSIG5, which upregulates PEGs to optimize photosystem repair .
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Reduces chloroplast oxidative damage by balancing PSI/PSII ratios .
Unresolved Questions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- ATHB17 can positively modulate the expression of numerous plastid-encoded genes (PEGs) through regulation of ATSIG5 by binding to their promoters. PMID: 28358040
- Expression of ATHB17Delta113 in maize results in increased ear size at silking. PMID: 24736658
- Research indicates that ATHB17 plays a role in abscisic acid (ABA) and water-stress responses. Notably, the effect of ATHB17 on seedling growth in the presence of ABA was observed only during the postgermination seedling stage. PMID: 23456334
- A functional genomics study identified ATHB17, a transcription factor belonging to the homeodomain-leucine zipper class II family, as a novel target for enhancing photosynthetic capacity. PMID: 24006420
