ATP5A1 Antibody
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Description
2.1. Cancer Biology
The ATP5A1 antibody has been instrumental in studying its role in cancer progression. For example:
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Cervical Cancer: ATP5A1 overexpression was shown to promote apoptosis and regulate alternative splicing of oncogenic genes (e.g., MMP2, MMP19) in HeLa cells, suggesting its tumor-suppressive potential .
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Clear-Cell Renal Cell Carcinoma (ccRCC): Low ATP5A1 expression correlated with poor prognosis and aggressive tumor behavior, with the antibody validating its downregulation in ccRCC cell lines .
2.2. Maternal Immunity
In zebrafish, ATP5A1 exhibits antibacterial activity, protecting embryos from pathogens like Aeromonas hydrophila. The antibody confirmed its presence in embryonic lysates and demonstrated its role in membrane depolarization/permeabilization .
2.3. Mitochondrial Function
The antibody serves as a loading control for mitochondrial proteins in studies investigating Complex V activity. For instance, it was used to normalize mitochondrial protein levels in colon cancer cells treated with SIRT3 inhibitors .
3.2. Mechanistic Insights
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Alternative Splicing: ATP5A1 regulates splicing of genes involved in glucose metabolism and HIF-1 signaling, impacting cancer progression .
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Wnt/β-Catenin Pathway: Its downregulation in ccRCC correlates with pathway activation, suggesting a suppressive role .
3.3. Species-Specific Functions
While ATP5A1’s role in mitochondrial ATP synthesis is conserved, its immune function in zebrafish embryos underscores evolutionary divergence in its biological roles .
Published Applications and Validation
The antibody has been validated in 126 WB, 4 IHC, and 19 IF studies across diverse tissues and cell lines (e.g., 786-O, CAKI-1 renal cancer cells) . Its specificity is further supported by co-immunoprecipitation (CoIP) assays targeting mitochondrial complexes .
Product Specs
Buffer
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. Store at -20°C. Avoid freeze/thaw cycles.
Lead Time
Typically, we can ship the products within 1-3 business days after receiving your order. Delivery time may vary depending on the purchasing method or location. Please consult your local distributors for specific delivery timeframes.
Synonyms
ATP synthase alpha chain antibody; ATP synthase alpha chain; mitochondrial antibody; ATP synthase subunit alpha antibody; ATP synthase subunit alpha mitochondrial antibody; ATP synthase; H+ transporting; mitochondrial F1 complex; alpha subunit 1; cardiac muscle antibody; ATP synthase; H+ transporting; mitochondrial F1 complex; alpha subunit; 1 antibody; ATP synthase; H+ transporting; mitochondrial F1 complex; alpha subunit; isoform 1; cardiac muscle antibody; ATP synthase; H+ transporting; mitochondrial F1 complex; alpha subunit; isoform 2; non-cardiac muscle-like 2 antibody; ATP sythase (F1 ATPase) alpha subunit antibody; ATP5A antibody; Atp5a1 antibody; ATP5AL2 antibody; ATPA_HUMAN antibody; ATPM antibody; Epididymis secretory sperm binding protein Li 123m antibody; hATP1 antibody; HEL-S-123m antibody; MC5DN4 antibody; mitochondrial antibody; Mitochondrial ATP synthetase antibody; Mitochondrial ATP synthetase oligomycin resistant antibody; Modifier of Min 2 antibody; Modifier of Min 2 mouse homolog antibody; Modifier of Min 2; mouse; homolog of antibody; MOM2 antibody; OMR antibody; ORM antibody; OTTHUMP00000163475 antibody
Target Names
Uniprot No.
Target Background
Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) is responsible for generating ATP from ADP in the presence of a proton gradient across the mitochondrial membrane. This gradient is established by electron transport complexes of the respiratory chain. F-type ATPases consist of two distinct structural domains: F(1), containing the extramembraneous catalytic core, and F(0), containing the membrane proton channel. These domains are interconnected by a central stalk and a peripheral stalk. During ATP synthesis, the rotary mechanism of the central stalk subunits couples proton translocation to ATP synthesis in the catalytic domain of F(1). Subunits alpha and beta form the catalytic core within F(1). The rotation of the central stalk against the surrounding alpha(3)beta(3) subunits drives the hydrolysis of ATP at three separate catalytic sites located on the beta subunits. Subunit alpha does not possess the catalytic high-affinity ATP-binding sites. ATP5A1 has been shown to bind the bacterial siderophore enterobactin and may facilitate the mitochondrial accumulation of enterobactin-derived iron ions.
Gene References Into Functions
- Utilizing systems biology techniques to analyze gene coexpression networks, ATP5A1 was identified as highly expressed in normal kidney tissues compared to clear cell renal cell carcinoma (ccRCC) tissues across various grades. Functional and pathway enrichment analysis revealed that ATP5A1 is overrepresented in the oxidative phosphorylation pathway, which has been linked to tumorigenesis and tumor progression. PMID: 29207195
- This cohort study demonstrated that the ATP5A1 gene exhibited downregulation in patients with idiopathic Parkinson disease compared to control subjects. PMID: 28916538
- These findings highlight a critical role of the P2Y1/PI3Kbeta axis in endothelial cell proliferation, downstream of ecto-F1-ATPase activation by apoA-I. Targeting this pathway pharmacologically could offer a promising approach to enhance vascular endothelial protection. PMID: 28578353
- These results, combined with previously reported inhibition of respiratory complex I, indicate that the suppression of oxidative phosphorylation enzyme activity contributes to the cell growth inhibitory effect of ATRA. PMID: 27856255
- Elevated mRNA levels of ATP5A1 are associated with glioblastoma. PMID: 26526033
- Hemoglobin has been identified as a novel ligand of hepatocyte ectopic F1-ATPase. PMID: 26769832
- Our research found that ATP synthase subunit alpha (ATP5A) undergoes O-GlcNAcylation at Thr432. Notably, ATP5A O-GlcNAcylation was reduced in the brains of Alzheimer's disease patients and a transgenic mouse model. PMID: 26358770
- Mitochondrial calpain-1 disrupts ATP synthase, leading to mitochondrial reactive oxygen species generation, which promotes proinflammatory responses and myocardial dysfunction during endotoxemia. PMID: 26246018
- Studies suggest that the F-ATP synthase can reversibly transition into a channel that mediates permeability transition in a Ca(2+)-dependent manner. PMID: 25999424
- A mutation in ATP5A1 has been linked to a fatal neonatal mitochondrial encephalopathy. PMID: 23599390
- Research indicates that F1-ATPase (F1) is a rotary motor protein driven by ATP hydrolysis. The minimum complex of F1 required for its function as a rotary motor is the alpha3beta3gamma subcomplex. PMID: 23395605
- Homozygous mutations in this gene are likely the cause of mitochondrial disease in two sisters, supported by findings from a yeast model. PMID: 23596069
- F1-ATPase located on the cell surface of colonic epithelial cells plays a role in mediating cell proliferation. PMID: 23055519
- An interactive proteomics study investigated proteins that bind heterocomplexes with ABCC1 using coimmunoprecipitation and MS/MS analyses. The study found that ATP synthase alpha binds to ABCC1 in plasma membranes with a ratio of 2:1. PMID: 22188235
- Antibodies targeting the beta- and gamma-subunits of F(1)-ATPase are additional antimitochondrial antibodies found in primary biliary cirrhosis. PMID: 22098431
- A specific Cys residue in ATP synthase alpha subunit is targeted by multiple oxidative posttranslational modifications, suggesting that this residue may function as a redox sensor regulating ATP synthase activity. PMID: 21817160
- Data suggests a correlation between phosphate binding and the tightening of the alphabeta-interface at the F1-ATPase catalytic site. PMID: 21481781
- This protein has been found to be differentially expressed in the anterior cingulate cortex of male patients with schizophrenia. PMID: 20381070
- The alpha-chain of ATP synthase is implicated in neurofibrillary degeneration associated with Alzheimer's disease, characterized by the cytosolic accumulation of this mitochondrial protein, which belongs to the mitochondrial respiratory system. PMID: 12614671
- Within the structurally-confined internal aqueous cavity of the F1-motor of ATP synthase, function arises from free energy changes that shift the equilibrium between interfacial charge hydration and interfacial hydrophobic hydration. PMID: 16378738
- Analysis of vascular endothelial ectoadenylate kinase and plasma membrane ATP synthase. PMID: 16714292
- Higher levels of ATP5a1 expression are linked to specific Single Nucleotide Polymorphisms and TP53 mutation. PMID: 19261598
- These findings indicate that the linoleic acid-phospholipid-induced stimulation of hepatic HDL secretion is associated with the expression and function of membrane ATP metabolizing proteins. PMID: 19717637
- This protein has been found to be differentially expressed in the Wernicke's Area of patients with schizophrenia. PMID: 19405953
- This protein has been found to be differentially expressed in the dorsolateral prefrontal cortex of patients with schizophrenia. PMID: 19110265
Database Links
Involvement In Disease
Combined oxidative phosphorylation deficiency 22 (COXPD22); Mitochondrial complex V deficiency, nuclear 4 (MC5DN4)
Protein Families
ATPase alpha/beta chains family
Subcellular Location
Mitochondrion. Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Cell membrane; Peripheral membrane protein; Extracellular side.
Tissue Specificity
Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
Customer Reviews
,
B.A
By Anonymous
★★★★★
Applications : Western blot
Sample type: tissues
Review: anti ATP5F1A was obtained from CUSABIO.(Complex V, PA002344HA01HU; CUSABIO, China).
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