ATP5D Antibody Pair
Description
Product Overview
The ATP5D Antibody Pair is primarily marketed by Cusabio for use in Sandwich Enzyme-Linked Immunosorbent Assays (S-ELISA). This system employs two antibodies:
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Capture Antibody (CSB-EAP00635C): A rabbit-derived IgG polyclonal antibody that binds to specific epitopes of ATP5D.
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Detection Antibody (CSB-EAP00635D): A biotinylated rabbit IgG antibody used for signal amplification via streptavidin-horseradish peroxidase (SA-HRP) conjugates.
| Parameter | Value |
|---|---|
| Reactivity | Human, guinea pig, bovine, horse, pig, goat |
| Concentration (Capture) | 0.5 μg/mL (recommended) |
| Concentration (Detection) | 0.25 μg/mL (recommended) |
| Plate Capacity | ≥5 × 96-well plates |
| Format | Liquid (PBS buffer) |
This antibody pair is optimized for detecting ATP5D in mitochondrial extracts, with applications spanning basic research and diagnostic assays .
Research Context
ATP5D is a 17 kDa subunit of the mitochondrial F1Fo-ATP synthase complex, where it stabilizes the enzyme’s structure and facilitates proton translocation for ATP synthesis . Recent studies highlight its role in cancer metabolism:
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m1A RNA Modification: ATP5D expression is regulated by m1A RNA methylation, which enhances glycolysis in cancer cells via YTHDF1-mediated translation .
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Therapeutic Targeting: Knockdown of ALKBH3 (an m1A demethylase) increases ATP5D levels, suggesting its potential as a metabolic target in oncology .
The ATP5D Antibody Pair enables quantitative analysis of these pathways, with applications in:
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Cancer Biology: Assessing ATP5D levels in tumor tissues or cell lysates.
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Mitochondrial Function: Monitoring ATP synthase activity in metabolic disorders .
Technical Considerations
Product Specs
**Detection:** 50% Glycerol, 0.01M PBS, pH 7.4
The ATP5D Antibody Pair is designed for use in S-ELISA applications to detect the ATP synthase subunit delta, a mitochondrial protein essential for ATP production. This antibody pair exhibits reactivity across a range of species including human, guinea pig, bovine, horse, pig, and goat. The ATP5D Antibody Pair comprises two antibodies: the capture antibody (CSB-EAP00635C) and the detection antibody (CSB-EAP00635D).
Both antibodies are rabbit-derived and supplied in liquid form, with the detection antibody conjugated with biotin for signal amplification. The recommended concentration for the capture antibody is 0.5 μg/mL, while the detection antibody is recommended at 0.25 μg/mL. Optimal antibody dilutions may vary depending on the specific experimental conditions. This Antibody Pair provides sufficient reagents to support at least 5 x 96 well plates when adhering to the recommended protocol.
