ATP6V1C1 Antibody

Introduction

The ATP6V1C1 antibody is a polyclonal reagent designed to detect the ATP6V1C1 protein, a critical subunit of vacuolar ATPase (V-ATPase), an enzyme responsible for acidifying intracellular compartments such as lysosomes and the Golgi apparatus. This antibody is widely used in research to study the protein’s role in cellular processes, including bone resorption, cancer progression, and lysosomal function.

Structure and Function of ATP6V1C1

ATP6V1C1 is a 382-amino acid protein (molecular weight ~44 kDa) that functions as the C1 subunit of the V1 domain of V-ATPase. It interacts with other subunits (e.g., ATP6V0A3) to regulate proton pump activity and cellular acidification . The antibody targets epitopes within the protein’s C-terminus or full-length sequence, enabling its detection in human, mouse, and rat tissues .

Applications of ATP6V1C1 Antibody

The antibody is validated for multiple techniques:

• Western Blot (WB): Detects ATP6V1C1 in lysates of human brain, testis, and cancer cell lines (e.g., HeLa) .

• Immunohistochemistry (IHC): Localizes the protein in tissues like human stomach cancer, kidney, and cerebellum .

• Immunofluorescence (IF): Visualizes ATP6V1C1 in cellular compartments, including lysosomes and the plasma membrane .