AZG2 Antibody
Description
AZG2 Gene and Protein Overview
AZG2 encodes a transporter protein belonging to the xanthine/uracil permease family. Key features include:
AZG2 facilitates cytokinin (CK) diffusion, influencing lateral root (LR) development and auxin-CK crosstalk . Its expression is restricted to cells surrounding lateral root primordia (LRP) .
Functional Significance of AZG2 in Plant Biology
AZG2 regulates hormone signaling pathways:
Role in Cytokinin Transport
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CK Accumulation: AZG2 transports CKs (e.g., tZ) with high efficiency in yeast and Arabidopsis .
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Subcellular Localization: ER localization suggests CK redistribution to intracellular compartments, potentially modulating receptor signaling .
Auxin-Cytokinin Interactions
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Lateral Root Development: AZG2 loss-of-function mutants show reduced LR formation, indicating a role in CK supply to auxin-responsive tissues .
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Reporter Gene Studies: AZG2 activity correlates with reduced TCSnpro:GFP (CK reporter) signal in mutant roots .
Potential Applications for AZG2 Antibodies
While no AZG2-specific antibodies are documented, general antibody strategies for transporter proteins can be extrapolated:
Antibody Design and Production
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Epitope Selection: Target conserved regions in AZG2’s transmembrane domains (TMDs) or cytosolic loops.
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Host Systems: Use E. coli or yeast for recombinant AZG2 production (e.g., GST-tagged fusions) .
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Purification: Affinity chromatography (e.g., Ni-NTA for His-tagged proteins) .
Validation Techniques
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Western Blotting: Detect AZG2 in membrane-enriched fractions (e.g., PM vs. ER-isolated vesicles) .
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Immunoprecipitation: Confirm antibody specificity by pull-down assays with AZG2-overexpressing lines .
Comparative Analysis of AZG1 and AZG2
AZG2 differs functionally from its paralog AZG1:
