GALT1 Antibody
Description
Introduction to GALT1 Antibody
The GALT1 Antibody, also known as the galactose-1-phosphate uridylyltransferase antibody, is a tool used in research to detect and study the GALT enzyme. This enzyme plays a crucial role in the metabolism of galactose, a sugar found in milk and other dairy products, through the Leloir pathway. The GALT enzyme catalyzes the conversion of UDP-glucose and α-D-galactose 1-phosphate into α-D-glucose 1-phosphate and UDP-galactose, which is essential for energy production and the synthesis of glycoproteins and glycolipids .
Types of GALT1 Antibodies
GALT1 antibodies are available in various forms, including monoclonal and polyclonal antibodies, which can be used for different applications such as Western blotting, immunoprecipitation, immunofluorescence, and enzyme-linked immunosorbent assay (ELISA).
-
Monoclonal Antibodies: These are highly specific and can be produced in large quantities. An example is the mouse monoclonal IgG1 kappa light chain antibody available from Santa Cruz Biotechnology, which detects human GALT protein .
-
Polyclonal Antibodies: These offer broader reactivity and are often used when specificity is less critical. An example is the rabbit polyclonal antibody available from Proteintech, which targets GALT in human samples .
Applications of GALT1 Antibodies
GALT1 antibodies are used in various research applications:
Research Findings and Clinical Significance
The GALT enzyme is crucial for galactose metabolism. Mutations in the GALT gene can lead to galactosemia, a metabolic disorder that can cause severe health issues if not properly managed . Research using GALT1 antibodies helps in understanding the role of GALT in disease pathology and in developing diagnostic tools.
References Santa Cruz Biotechnology. GALT Antibody (G-1): sc-365577. Induction of Cytolytic Anti-Gal Antibodies in α-1,3-Galactosyltransferase Knockout Mice. Generation and characterization of a monoclonal antibody that binds to Galectin-1. Abcam. Anti-GALT antibody [EPR12555]. The Formation of Glycan-Specific Natural Antibodies Repertoire in GalT-KO Mice. Improvement of Mutant Galactose-1-Phosphate Uridylyltransferase (GALT) Activity by FDA-Approved Pharmacochaperones. Proteintech. GALT antibody (17035-1-AP).
Product Specs
Components: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
GALT1's Role in Lewis a Structure Biosynthesis:
Research indicates that GALT1 is both necessary and sufficient for the addition of β1,3-linked galactose residues to N-glycans. This enzymatic activity is therefore essential for the biosynthesis of Lewis a structures in Arabidopsis. (PMID: 17630273)
