The At4g14600 Antibody (Catalog Code: CSB-PA837770XA01DOA) is a polyclonal antibody developed for research applications targeting the At4g14600 protein in Arabidopsis thaliana (mouse-ear cress). This antibody is part of a broader portfolio of plant-specific reagents designed to study protein localization, function, and interactions in plant biology. Below is a detailed breakdown of its specifications and potential applications, synthesized from available data.
While no peer-reviewed studies explicitly detail the use of the At4g14600 Antibody, its development aligns with broader trends in plant antibody research. Arabidopsis antibodies are critical for:
Protein localization studies: Immunofluorescence or immunoblotting to map protein distribution in tissues like pollen tubes or root cells.
Functional studies: Knockdown or overexpression experiments to assess phenotypic changes (e.g., root growth, stress responses) .
Interaction mapping: Co-immunoprecipitation (Co-IP) to identify binding partners in cellular pathways.
For example, antibodies targeting syntaxins (e.g., AtSYP31/32) have been used to study pollen wall development, vesicle trafficking, and cell wall integrity in Arabidopsis . While not directly involving At4g14600, these studies illustrate the methodologies applicable to similar antibodies.
Key limitations include:
No Published Validation Data: The provided sources do not include immunoblot, immunofluorescence, or ELISA validation for the At4g14600 Antibody.
Functional Context Unclear: The gene At4g14600 encodes a hypothetical protein with limited annotated functional data in public databases (e.g., TAIR, UniProt).
Species Specificity: The antibody is described for Arabidopsis only; cross-reactivity with other plant species is unverified.