BGAL5 Antibody

Biochemical Function of BGAL5

B4GALT5 catalyzes the synthesis of lactosylceramide (LacCer) by transferring galactose from UDP-galactose to glucosylceramide (GlcCer) . LacCer serves as the precursor for gangliosides, which are essential for neuronal maturation, axonal guidance, and myelin formation . Additionally, BGAL5 regulates BMPR1A glycosylation, influencing protein stability and embryonic development .

Glycosphingolipid Biosynthesis Studies

BGAL5 antibodies enable the detection of B4GALT5 expression in tissues, particularly in studies investigating lactosylceramide’s role in neural development and cancer progression . For example, LacCer synthesis by BGAL5 is critical for ganglioside diversity in the central nervous system .

Disease Models

• Cancer: Aberrant glycosylation mediated by BGAL5 has been implicated in tumor cell adhesion and signaling. Antibodies against BGAL5 help identify glycosylation patterns in cancer-specific glycopeptides .

• Developmental Biology: BGAL5 knockout models reveal its necessity in extraembryonic tissue development, with antibodies used to validate protein expression in embryogenesis studies .

Advanced Characterization Techniques

Recent studies employ high-resolution methods like cryo-electron microscopy polyclonal epitope mapping (cryoEMPEM) to analyze antibody-antigen interactions . While not directly applied to BGAL5 yet, such techniques could refine epitope mapping for BGAL5 antibodies in future research .