BGLU21 Antibody

Metabolomic Analysis

Comparative studies between wild-type and bglu23 bglu21 double mutants revealed BGLU21’s role in glucosinolate metabolism:

Data from seedling homogenates incubated at 26°C .

• Key Insight: BGLU21 deficiency disrupts glucosinolate hydrolysis, rendering plants susceptible to herbivory (e.g., woodlice preferentially consumed bglu23 bglu21 mutants) .

Protein Interaction Studies

BGLU21 antibodies have been instrumental in co-immunoprecipitation assays to study protein interactions:

• NAI2-BGLU23 Complex: Anti-GFP antibodies immunoprecipitated NAI2-GFP alongside BGLU23, confirming their interaction .

• Antibody Specificity: Polyclonal antibodies raised against recombinant BGLU fragments enabled precise detection in immunoblots .

BGLU21 Antibody Development

BGLU21 antibodies are typically generated using recombinant protein fragments. For example:

• Antigen Design: A partial cDNA encoding amino acids 272–502 of BGLU21 is cloned, expressed in E. coli, and purified via nickel-column chromatography .

• Immunization: Purified proteins are injected into rabbits to produce polyclonal antibodies, followed by affinity purification .

Applications of BGLU21 Antibody

• Immunoblotting: Detects BGLU21 expression levels in plant tissues under stress conditions .

• Co-Immunoprecipitation: Identifies interaction partners (e.g., NAI2) in ER-body protein complexes .

• Localization Studies: Tracks BGLU21 sequestration in ER bodies versus leakage into vacuoles during defense responses .

Technical Considerations

• Cross-Reactivity: BGLU21 antibodies may cross-react with BGLU23 due to structural homology; specificity must be validated via mutant controls .

• Functional Assays: Antibodies are critical for validating bglu21 knockout lines and assessing glucosinolate defense efficacy .