BGLU46 Antibody
Description
Introduction to BGLU46 Antibody
The BGLU46 antibody is a specific tool used in research to detect the β-glucosidase 46 (BGLU46) protein. BGLU46 is an enzyme involved in the hydrolysis of monolignol glucosides, which are crucial intermediates in the lignification process of plants. This enzyme, along with its closely related counterpart BGLU45, plays a significant role in the phenylpropanoid pathway, particularly in the metabolism of coniferin, a glucoside of coniferyl alcohol .
Function and Localization of BGLU46
BGLU46 is primarily localized in the protoxylem of plant stems, as demonstrated by immunolocalization studies using specific antibodies . The enzyme is secreted to the cell wall, where it participates in the hydrolysis of monolignol glucosides. Although BGLU46 is involved in the phenylpropanoid pathway, knockout mutants of the BGLU46 gene do not exhibit a lignin-deficient phenotype, suggesting that its role in lignification is not critical or is compensated by other enzymes .
Expression and Activity
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Expression: BGLU46 is expressed in the protoxylem of mature stems, as shown by in situ hybridization experiments .
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Activity: The enzyme is specific for certain monolignol glucosides, although its substrate specificity is somewhat different from that of BGLU45 .
Mutant Studies
Knockout mutants of BGLU46 have been generated to study its function. These mutants do not display visible phenotypic changes under normal growth conditions, indicating that BGLU46's role might be redundant or not essential for plant development .
Data Tables
The following table summarizes the characteristics of BGLU46 mutants:
| Gene | Arabidopsis Genome Initiative No. | Flanking Sequence Tag | Mutant Name | T-DNA Insertion | RT-PCR | Accession |
|---|---|---|---|---|---|---|
| GLU 46 | At1g61820 | FLAG_267A12 | bglu46-1 | 5′ untranslated region | Overexpressor | WS |
| FLAG_395C03 | bglu46-2 | Intron 5 | Null | WS | ||
| GABI 295C07 | bglu46-3 | Intron 5 | Null | Col-0 |
