BHLH10 Antibody
Description
Introduction to BHLH10 Antibody
The term "BHLH10 Antibody" can refer to antibodies targeting different proteins, depending on the context. BHLH10 is a basic helix-loop-helix transcription factor involved in various biological processes. Therefore, an antibody against BHLH10 can be utilized in diverse research areas.
HOXD10 Antibody
One possible target for a BHLH10 antibody is the HOXD10 protein.
2.1. General Information
The Human HOXD10 Antibody (AF10413) is an antibody developed by R&D Systems that targets the human HOXD10 protein . HOXD10 is a transcription factor involved in cell survival, apoptosis, migration, and invasion . Re-expression of HOXD10 can significantly inhibit cell survival, induce cell apoptosis, and impair cell migration and invasion .
2.2. Antibody Details
| Attribute | Description |
|---|---|
| Source | E. coli-derived recombinant human HOXD10 |
| Amino Acid Sequence | Arg153-Thr262 |
| Accession Number | P28358 |
2.3. Applications
The Human HOXD10 Antibody can be used in various applications, including the fluorescent ICC staining of cells on coverslips . Optimal dilutions should be determined by each laboratory for each application .
2.4. Example of Usage
In a study, the antibody was used at a concentration of 10 µg/mL to detect HOXD10 in human induced pluripotent stem cells (iPS) differentiated into neural progenitor cells (NPC) . The cells were co-stained with a Mouse Anti-Human Nestin Monoclonal Antibody (Catalog # MAB1259) at 10 µg/mL . Specific staining for HOXD10 was localized to the nucleus, while Nestin co-staining was cytoplasmic .
BHLH34 and BHLH104 Antibodies
In Arabidopsis thaliana, BHLH34 and BHLH104 are two bHLH transcription factors that regulate iron (Fe) homeostasis .
3.1. Function
These transcription factors positively regulate Fe homeostasis by activating the transcription of the Ib subgroup bHLH genes . bHLH34 and bHLH104 directly activate the transcription of bHLH38/39/100/101 .
3.2. Interaction
Protein interaction assays indicate that both heterodimers and homodimers occur between bHLH34, bHLH104, and bHLH105 . bHLH104 interacts with ILR3 to regulate the Arabidopsis Fe deficiency responses via targeting the Ib subgroup bHLH genes .
3.3. Regulation of Gene Expression
bHLH104 and ILR3 regulate the expression of four Ib subgroup bHLH genes and PYE by binding to specific elements in their promoters, indicating a cascade of bHLH transcription factors during the control of Fe homeostasis . bHLH104 and ILR3 interact with the specific E-box regulatory elements of Ib subgroup bHLH genes and PYE to affect their transcription during the response to Fe deficiency .
Tissue Factor Antibody (10H10)
The murine antibody 10H10, raised against human tissue factor (TF), blocks the signaling pathway, inhibiting angiogenesis and tumor growth without interfering with coagulation .
4.1. Uniqueness
Unlike other anti-TF antibodies, mAb 10H10 specifically blocks the activation of PAR2 by the TF-FVII binary complex without inhibiting coagulation .
4.2. Humanization and Affinity
The antibody was humanized through a two-step procedure involving grafting antigen-binding loops onto selected human frameworks and affinity maturation using phage display libraries . The humanized mAbs were further characterized for their ability to recognize endogenous TF expressed on human breast cancer cells MDA-MB231, with all 10 variants exhibiting binding comparable to the parental mAb 10H10 .
H10-Directed Monoclonal Antibodies
H10-directed monoclonal antibodies (MAbs) display hemagglutination inhibition (HI) and neutralization activity against the H10N8 influenza virus .
5.1. Functionality
These antibodies can be used for the functional inhibition of hemagglutination and neutralization . Some HI-reactive H10 antibodies also demonstrated neuraminidase inhibition (NI) activity against H10N8 and an H10N7 strain, possibly through steric hindrance .
