BHLH79 Antibody
Description
Introduction to BHLH79 Antibody
The BHLH79 antibody targets a protein known as BHLH79, which belongs to the basic helix-loop-helix (bHLH) family of transcription factors . These proteins are characterized by a bHLH domain that facilitates DNA binding and protein dimerization, playing crucial roles in various biological processes, including cell differentiation, development, and responses to environmental stimuli .
Characteristics of BHLH79 Protein
BHLH79 proteins, as members of the bHLH family, typically function as transcription factors that regulate gene expression . They often form dimers with other bHLH proteins to bind to specific DNA sequences, thereby influencing the transcription of target genes.
Applications of BHLH79 Antibody
BHLH79 antibodies are valuable tools for:
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Studying Protein Expression: They can be used to detect the presence and levels of BHLH79 protein in different tissues or cell types .
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Analyzing Subcellular Localization: These antibodies can help determine where BHLH79 protein is located within cells .
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Investigating Protein Function: By blocking or detecting BHLH79, researchers can elucidate its role in various biological processes .
Research Findings Involving BHLH79 Antibody
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Cold Stress Response: In Brassica campestris, CabHLH79 enhances cold tolerance by upregulating the expression of genes related to reactive oxygen species (ROS) and other cold stress tolerance mechanisms .
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Immune Response to Tuberculosis: Research has identified human antibodies against Mycobacterium tuberculosis (MTB) antigens, revealing insights into the human B-cell response to MTB . These antibodies, including IgA isotypes, can block MTB uptake by lung epithelial cells .
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Cancer Immunotherapy: Identification of immunogenic epitopes that bind to the major histocompatibility complex (MHC) is critical for personalized cancer vaccines and cell therapies .
Methodologies Using BHLH79 Antibody
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Western Blotting: Used to confirm the specificity of antibodies by identifying target proteins based on their molecular weight .
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ELISA (Enzyme-Linked Immunosorbent Assay): Employed to detect and quantify the interaction between antibodies and antigens .
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Flow Cytometry: Used to assess antibody binding to cells, helping to identify and sort cell populations based on BHLH79 expression .
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Immunofluorescence Microscopy: Helps visualize the localization of BHLH79 protein within cells and tissues .
Data Tables
Because the nature of BHLH79 antibody research spans multiple fields, the following tables exemplify the varied data it can generate.
Table 1: Antibody Specificity Data
| Antibody ID | Target Antigen | Reactivity | Cross-Reactivity | Application |
|---|---|---|---|---|
| BHLH79-Ab01 | BHLH79 | Positive | None Reported | Western Blot, IF |
| BHLH79-Ab02 | BHLH79 | Positive | Other bHLH | ELISA, Flow |
| CS-35 | MTB ManLAM | Positive | Nonpathogenic Mycobacterium smegmatis | Western blot, FACS, and ELISA |
Table 2: Functional Assay Data in Cold Stress Response
| Gene | Treatment | Expression Level | Change Compared to Control |
|---|---|---|---|
| ROS-Related | Cold Stress | High | Significantly Increased |
| COR15A | Cold Stress | Elevated | Increased |
Table 3: Immunogenicity Scores of MHC Epitopes
| HLA Class | Immunogenicity Score Distribution |
|---|---|
| Class I | Normal Distribution |
| Class II | Skewed Towards Upper Limit |
Product Specs
Target Background
Q&A
The query appears to reference a typographical error ("BHLH79" instead of "BHLHB9"), a basic helix-loop-helix transcription factor. Below are research-focused FAQs with methodological guidance, organized by complexity and supported by experimental evidence from peer-reviewed studies.
Basic: What experimental controls are essential for BHLHB9 functional studies?
Protocol:
Advanced: How to resolve contradictory data in BHLHB9 subcellular localization studies?
Analysis Framework:
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Technical variables: Compare fixation methods (e.g., PFA vs. methanol) and antibody dilution gradients .
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Biological context: Assess cell cycle-dependent localization using synchronized cultures .
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Orthogonal validation: Combine ICC/IF with subcellular fractionation followed by Western blot .
Advanced: What computational tools predict BHLHB9-antibody interactions for rational epitope design?
Approach:
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Use IgDiff (SE(3) diffusion model) to generate novel CDR-H3 loops with RMSD <1.5Å from natural antibodies .
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Apply MAMMAL framework to predict binding activity (AUROC=0.73 in mAb-exclusive splits) .
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Validate using protein microarrays (>9,000 human proteins) to detect off-target interactions .
Advanced: How to optimize BHLHB9 antibody performance in multiplexed assays?
Experimental Design:
Advanced: What metrics differentiate high-quality BHLHB9 antibodies for structural studies?
Quality Criteria:
| Metric | Threshold | Method |
|---|---|---|
| Designability | scRMSD <2Å in CDR loops | IgDiff inpainting |
| Affinity | KD <10 nM | BLI/SPR with recombinant BHLHB9 |
| Thermal stability | Tm >65°C | DSF/TSA |
Key Findings from Literature:
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Autoreactivity risk: 30% of stem-targeting influenza bNAbs show pituitary gland cross-reactivity , suggesting similar screening for BHLHB9 antibodies.
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Expression success: SE(3)-designed antibodies achieve 100% expressibility in HEK293 .
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Data robustness: HA-exclusive splits in ML models yield AUROC=0.90 for antigen-antibody interaction prediction .
