BIN2 Antibody

Introduction to BIN2 Antibody

BIN2 antibodies are specialized immunoreagents targeting the BIN2 protein, a molecule with distinct biological roles in plants and mammals. These antibodies are critical tools for studying BIN2’s involvement in cellular signaling, structural dynamics, and disease mechanisms. This article synthesizes findings from peer-reviewed research and commercial antibody specifications to provide a comprehensive overview.

BIN2 Antibody in Plant Biology

2.1 Background on BIN2 in Plants
BIN2 (Brassinosteroid Insensitive 2) is a serine/threonine kinase in Arabidopsis thaliana that regulates brassinosteroid signaling, influencing plant growth and stress responses. It phosphorylates transcription factors like BZR1 and BZR2, promoting their degradation and modulating brassinosteroid-mediated gene expression .

Anti-BIN2 Antibody (Agrisera AS16 3203)

• Host: Rabbit (polyclonal)

• Reactivity: Arabidopsis thaliana (recombinant BIN2), with predicted cross-reactivity in Glycine max, Phaseolus vulgaris, and other dicots .

• Applications:

  • Western blot: 1:5,000 dilution detects recombinant BIN2-GST fusion protein (~69 kDa) .

  • Immunofluorescence: 1:100 dilution localizes BIN2 in plant tissues .

Research Findings

• BIN2 knockdown in Arabidopsis disrupts vacuole formation, impairing female gametophyte development .

• The antibody’s specificity was validated using recombinant BIN2-GST, showing linear detection sensitivity from 2.5–30 ng of protein .

BIN2 Antibody in Mammalian Systems

3.1 Background on Bin2 in Leucocytes
In mammals, Bin2 is a membrane-sculpting N-BAR protein expressed in leucocytes. It localizes to podosomes and phagocytic cups, regulating cell migration, actin dynamics, and phagocytosis .

Anti-Bin2 Antibody in Immune Research

• Target: Human Bin2 C-terminus (rabbit polyclonal) .

• Key Findings:

  • Bin2 siRNA knockdown reduces podosome density and monocyte migration but enhances macrophage phagocytosis .

  • Overexpression increases podosome density and dynamics .

Comparative Analysis

Key Research Insights

Table 1: Functional Roles of BIN2 Across Species