BMPER Antibody
Description
Definition and Purpose of BMPER Antibody
BMPER Antibody is a specialized immunological reagent designed to detect the bone morphogenetic protein (BMP) endothelial cell precursor-derived regulator (BMPER) protein. It facilitates the study of BMPER’s localization, expression levels, and functional interactions in biological systems. These antibodies are critical for understanding BMPER’s role in vascular development, inflammation, and disease pathologies.
Key applications include:
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Immunocytochemistry/Immunofluorescence: To map BMPER distribution in tissues or cells.
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Western Blotting: To quantify BMPER protein expression in lysates.
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Immunohistochemistry: To analyze BMPER in tissue sections.
Immunostaining in Developmental Biology
In embryonic studies, the Mouse Crossveinless-2/CV-2 Antibody (AF2299) from R&D Systems has been used to visualize BMPER in the aorta-gonad-mesonephros (AGM) region of mouse embryos . This antibody detects BMPER in perivascular cells and subaortic mesenchyme, showing spatial complementarity with phosphorylated Smad1/5/8 (pSMAD1/5/8), a marker of BMP signaling .
Key Findings:
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BMPER expression peaks in the AGM region during hematopoietic development.
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Localization patterns suggest BMPER negatively regulates BMP signaling via feedback inhibition .
Western Blotting in Disease Models
The Anti-BMPER Antibody (ab75183) from Abcam has been employed to study BMPER downregulation in renal fibrosis. In unilateral ureteral obstruction (UUO) models, Western blotting revealed reduced BMPER expression in kidneys and TGF-β1-treated HK-2 tubular cells .
Key Findings:
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BMPER levels inversely correlate with fibrosis markers (e.g., fibronectin, collagen I).
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Exogenous BMPER administration mitigates tubular dedifferentiation and fibroblast activation .
Vascular Development and Signaling Regulation
BMPER antibodies have clarified BMPER’s dual role as a BMP antagonist and modulator. For instance:
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Negative Feedback on BMP Signaling: AF2299 staining in mouse embryos demonstrated that BMPER-rich regions exhibit reduced pSMAD1/5/8, indicating suppression of BMP activity .
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Context-Dependent Activity: While BMPER inhibits BMP4 in some contexts, its loss exacerbates vascular inflammation by upregulating adhesion molecules (e.g., VCAM-1, ICAM-1) .
Therapeutic Potential in Fibrosis
In kidney fibrosis models, BMPER antibodies revealed its protective role:
| Model | BMPER Antibody Used | Observed Effect | Citation |
|---|---|---|---|
| UUO Mice | ab75183 (Abcam) | Reduced fibronectin and collagen I | |
| TGF-β1-Treated HK-2 Cells | ab75183 (Abcam) | Inhibited E-cadherin loss and Id1 upregulation |
Table 1: BMPER Antibodies in Research
| Antibody Name (Catalog Number) | Manufacturer | Species | Application | Key Features |
|---|---|---|---|---|
| Mouse Crossveinless-2/CV-2 (AF2299) | R&D Systems | Mouse | Immunocytochemistry, Immunofluorescence | Detects perivascular BMPER; quantifies pSMAD1/5/8 complementarity |
| Anti-BMPER (ab75183) | Abcam | Human | Western Blotting | Validates BMPER downregulation in fibrosis |
Table 2: Functional Insights from BMPER Antibody Studies
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Overexpression of BMPER significantly enhanced BMP-2-induced osteogenic differentiation, while suppression of BMPER effectively inhibited this process both in vitro and in vivo. PMID: 29518774
- BMPER modulation of BMP pathway activity regulates VE-cadherin expression and vascular barrier function. PMID: 27995357
- BMPER variants are associated with a novel, attenuated subtype of diaphanospondylodysostosis. PMID: 26467725
- A BMPER-dependent pathway is implicated in high glucose induced alkaline phosphatase expression in vascular smooth muscle cells. PMID: 26264461
- The proangiogenic effect of BMPER in endothelial cells is mediated by inhibition of antiangiogenic thrombospondin-1 and enhanced expression and activation of the FGF signaling pathway, which is crucial for promoting angiogenesis. PMID: 25503991
- BMPER serves as a novel regulator of the osteoblast-like differentiation of HCASMCs. PMID: 22778264
- Bmper plays a critical role in regulating Bmp-mediated vascular inflammation, and precise control of Bmp and Bmper levels is essential for maintaining normal vascular homeostasis. PMID: 22772758
- Research has definitively demonstrated that BMPER is highly expressed in malignant tumors and that the growth of lung, colon, and uterine carcinomas relies on the presence of BMPER. PMID: 22020334
- Mutual regulation by BMP-9 and CV2 is critical in regulating the development of the vascular endothelium. PMID: 22474252
- Loss of BMPER function is responsible for Diaphanospondylodysostosis. PMID: 21990102
- BMPER-mediated signaling plays a crucial role in vertebral segmentation early in human development, while defects in BMPER lead to Diaphanospondylodysostosis. PMID: 20869035
- BMPER, a novel BMP-binding protein, is expressed by endothelial cell precursors, exhibits BMP-antagonizing activity, and potentially contributes to endothelial cell differentiation by modulating local BMP activity. PMID: 12897139
- Crossveinless-2 acts as an inhibitor of BMP function. PMID: 14766204
- BMPER functions as an endothelial cell regulator and controls bone morphogenetic protein-4-dependent angiogenesis. PMID: 18787191
