tbxta Antibody
Description
Biological Role of TBXT
TBXT is a transcription factor encoded by the TBXT gene, crucial for:
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Embryogenesis: Directing mesoderm specification and notochord development during gastrulation .
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Cancer Biology: Acting as a driver in chordoma (a rare spinal cancer) and promoting epithelial-mesenchymal transition (EMT) in breast cancer .
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Regulatory Functions: Modulating gene networks involved in cell cycle control, DNA repair, and metabolic processes .
TBXT Antibody: Key Applications
TBXT antibodies are widely used in research for:
Role in Cancer
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Chordoma: TBXT is overexpressed in >90% of chordomas and sustains tumor cell survival. Antibodies helped identify TBXT-DNA interactions and fragment-based inhibitors .
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Breast Cancer: TBXT drives EMT and metastasis. Antibody-based assays revealed miR-219-5p suppresses TBXT, reducing invasion and stemness .
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Therapeutic Targeting: DARPins (designed ankyrin repeat proteins) blocking TBXT-DNA binding inhibit tumor growth in preclinical models .
Developmental Biology
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TBXT dosage controls EMT timing during gastrulation. Heterozygous (TBXT-Het) models show delayed EMT, highlighting its role in cell adhesion and migration .
Validation and Challenges
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Specificity: Polyclonal antibodies (e.g., Proteintech 20741-1-AP) target epitopes in the C-terminal region (residues 226-435) .
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Limitations: Commercial antibodies may exhibit cross-reactivity with paralogs (e.g., TBX6). Knockout validation is recommended .
Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Ntla and Spt do not exhibit differential regulation of medial floor plate (MPF) induction along the anterior-posterior axis. Instead, these T-box genes function redundantly within margin-derived cells to promote the posterior extension of MFP progenitors. PMID: 25781211
- Observations revealed normal cardiac convergence in spt(-/-) and ntl(-/-) embryos, suggesting that the novel roles of spt and ntl in PGP migration cannot be attributed solely to generalized effects on midline convergence. PMID: 23860396
- As a proof of concept, the study dynamically profiled No tail a (Ntla)-dependent genes at different stages of axial mesoderm development in zebrafish, identifying distinct sets of transcripts. PMID: 22286130
- Data demonstrate that embryonic mesodermal progenitors uniquely establish their own niche, with Brachyury being essential for creating a domain of high Wnt and low RA signaling, rather than relying on a niche created by separate support cells. PMID: 21159819
- The findings demonstrate that the nodal-independent regulation of ntl is crucial for tail formation in zebrafish. PMID: 20215349
- ntl function is required to repress medial floor plate and promote notochord fate in cells of the wild-type notochord domain. Conversely, spt and ntl, acting together non-cell autonomously, are required for medial floor plate formation. PMID: 12091302
- The intronless ntl gene, ctl, has a physiological function in zebrafish. PMID: 12804607
- The study explores the interplay between FGF, one-eyed pinhead (zoep), and T-box transcription factors spt and ntl during zebrafish posterior development. PMID: 14651930
- The no tail gene (ntl), a key gene involved in the formation of a normal tail, was employed as a target for ribonuclease (RNase) P-mediated gene disruption in zebrafish in vivo. PMID: 17294249
- The study investigates whether loss of Brachyury/Notail or Tbx16/Spadetail disrupts the balance of Nodal ligands (Southpaw) and antagonists (Charon) around Kupffers Vesicle. PMID: 17654709
- Ntl is essential for KV lumen formation. PMID: 17765888
- The endoplasmic reticulum Ca(2+) pump plays a role in the expression of no tail (ntl) and left-right dynein related (lrdr) in the dorsal forerunner cells during gastrulation. PMID: 18359204
- Regulation of canonical Wnt signaling by ntl is crucial for posterior mesoderm formation. PMID: 18606146
- Ntl regulates a downstream network of other transcription factors and identifies an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. PMID: 19225104
