BRX Antibody
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Description
BRX Antibodies (Targeting CHIC1/BRX Protein)
CHIC1/BRX is a 224-amino acid cytoplasmic protein with palmitoylation modifications . Antibodies against this protein are used in:
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Applications: ELISA, Western blot, immunocytochemistry, immunofluorescence, and immunohistochemistry .
Table 2: Anti-BRIX1 Antibody Details
| Parameter | Specification |
|---|---|
| Form | Buffered aqueous glycerol |
| Storage | −20°C |
| Validation | Human Protein Atlas protocols |
Brx Gene and Osmotic Stress in Immune Cells
While not an antibody, Brx (AKAP13) is a guanine nucleotide exchange factor (GEF) critical for immune cell responses to osmotic stress . Key findings:
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Mechanism: Activates p38 MAPK via interaction with JIP4 scaffold protein, inducing nfat5 expression and BAFF cytokine production .
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Functional Defects: Brx haploinsufficient mice show reduced IgG1 production and altered B cell differentiation .
Table 3: Brx Gene Functions
| Function | Experimental Evidence |
|---|---|
| Osmotic Stress Signaling | Hyperosmolarity triggers nfat5 upregulation via p38 MAPK |
| Humoral Immunity | Brx +/− mice exhibit impaired BAFF expression and IgG1 production |
Research Challenges and Validation
Antibody characterization remains critical for reproducibility. Programs like the Human Protein Atlas rigorously validate antibodies using:
Product Specs
Buffer
Preservative: 0.03% ProClin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
14-16 weeks (Made-to-order)
Synonyms
BRX antibody; At1g31880 antibody; F5M6.11Protein BREVIS RADIX antibody; AtBRX antibody
Target Names
BRX
Uniprot No.
Target Background
Function
BRX (Brevis Radix) is a key regulator of cell proliferation and elongation in plant roots and shoots. It plays a crucial role in root architecture development and primary root protophloem differentiation. BRX, in conjunction with BAM3 and CLE45, regulates the transition of protophloem cells from proliferation to differentiation, thereby influencing the postembryonic growth capacity of the root meristem. Functionally, BRX is a probable transcription regulator, regulated by the auxin response factor ARF5. Its polar localization within vascular cells and its involvement in endocytic recycling highlight its dynamic nature. BRX is essential for correct CPD expression and nuclear auxin response, mediating crosstalk between auxin and brassinosteroid pathways. Furthermore, it is a target for auxin-induced, proteasome-mediated degradation.
Gene References Into Functions
- A novel brx allele from an acidic soil environment demonstrates the presence of independently maintained loss-of-function alleles in nature, suggesting a selective advantage under acidic conditions. (PMID: 23041192)
- Research indicates a ubiquitous, albeit quantitatively variable, role for BRX in modulating growth rates in both roots and shoots. (PMID: 20649916)
- The AtBRX(Lc-0) allele contributes to natural variation in root growth vigor, representing a rare example of an experimentally confirmed hyperactive allelic variant. (PMID: 20404146)
- The brx phenotype arises from a root-specific brassinosteroid deficiency due to reduced BRX-dependent expression of a rate-limiting enzyme in brassinosteroid biosynthesis. (PMID: 17006513)
- A naturally occurring brx loss-of-function allele results in a root system characterized by increased branching but impaired elongation. (PMID: 18627499)
- BRX is implicated in cytokinin-mediated inhibition of lateral root initiation. (PMID: 19037657)
- A loss-of-function allele, brx-2, exhibits an enhanced response to ABA-mediated inhibition of root growth. (PMID: 19201913)
- BRX may synchronize cellular growth with the auxin concentration gradient at the root tip. (PMID: 19465596)
Database Links
Protein Families
BRX family
Subcellular Location
Nucleus. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue Specificity
Expressed in the developing protophloem up to the elongation zone in root meristem of young seedlings, in the columella and the phloem vasculature throughout the root and in the phloem vasculature in the shoot. Detected in the shoot meristem and in primor
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