BUB3.3 Antibody
Description
BUB3 Protein Overview
BUB3, or budding uninhibited by benzimidazoles 3 homolog, is a protein involved in the mitotic checkpoint complex. It helps regulate the metaphase to anaphase transition by inhibiting the anaphase-promoting complex (APC/C) until chromosomes are correctly aligned at the metaphase plate . BUB3 contains WD40 repeats, which are important for protein-protein interactions .
BUB3 Antibodies
BUB3 antibodies are tools used in research to detect and study the BUB3 protein. These antibodies are available in various forms, including polyclonal and monoclonal types, and are suitable for different applications such as Western blot (WB), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA).
Types of BUB3 Antibodies
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Polyclonal Antibodies: These are produced by multiple B cells and recognize multiple epitopes on the antigen. An example is the rabbit polyclonal BUB3 antibody (27073-1-AP) from Proteintech, which reacts with human and rat samples .
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Monoclonal Antibodies: These are produced by a single clone of B cells and recognize a single epitope. An example is the rabbit recombinant monoclonal BUB3 antibody [EPR5319(2)] from Abcam, suitable for human samples .
Applications of BUB3 Antibodies
BUB3 antibodies are used in various research applications:
Research Findings
BUB3 plays a critical role in the spindle assembly checkpoint, ensuring accurate chromosome segregation. Disruption of the BUB3 gene in mice results in early embryonic lethality, highlighting its essential function in mitosis . In plants, BUB3.3 interacts with BMF3 to recruit CDC20 to unattached kinetochores, inhibiting anaphase progression until proper microtubule attachment is achieved .
