PYD3 Antibody

Functional Role of PYD3 in Inflammasome Regulation

PYD3 is implicated in NLRP3 inflammasome activation, a critical mediator of inflammatory responses. In diabetic models, PYD3 shRNA significantly reduced:

• Phosphorylated p38 MAPK levels ($p < 0.05$)

• NLRP3, ASC, and caspase-1 protein expression ($p < 0.001$)

• IL-1β and IL-18 secretion ($p < 0.01$)

Mechanistic Insights:

• PYD3 interacts with NLRP3 during the interphase of the cell cycle, enhancing inflammasome assembly .

• Knockdown of PYD3 disrupts pyroptosis (inflammatory cell death) by inhibiting Gasdermin D (GSDMD) cleavage .

PYD3 in β-Alanine Metabolism

In Drosophila, PYD3 regulates β-alanine recycling. Mutants exhibit:

• 270–300% increase in head β-alanine levels upon uracil exposure .

• Impaired histamine conjugation, affecting neurotransmitter recycling .

Data adapted from Drosophila studies .

Antibody Applications in PYD3 Research

While no commercial PYD3-specific antibody is explicitly documented, related antibodies are used to study pathways involving PYD3:

Key Antibodies in PYD3-Associated Pathways:

Experimental Workflow Example:

1. Cell Transfection: HEK293T cells transfected with NLRP3, ASC, pro-caspase-1, and pro-IL-1β .

2. Immunoblotting: Anti-caspase-1 (1:200 dilution) and anti-IL-1β antibodies detect cleavage events .

3. ELISA: Quantify mature IL-1β in supernatants .

Research Gaps and Future Directions

• Antibody Specificity: No validated PYD3 antibody is reported; most studies infer PYD3 activity via shRNA knockdown and downstream protein analysis .

• Therapeutic Potential: Targeting PYD3-NLRP3 interactions could mitigate diabetic neuropathy or inflammatory diseases .

Technical Considerations

• Primers for PYD3 Studies:

  Gene	Forward Primer	Reverse Primer
  NLRP3	5′-CTGCATGCCGTATCTGGTTG-3′	5′-CGGCGTTAGCAGAAATCCAG-3′
  ASC	5′-CTCTGTATGGCAATGTGCTGAC-3′	5′-GAACAAGTTCTTGCAGGTCAG-3′

• Statistical Analysis: Data analyzed via ANOVA ($p < 0.05$) and Student’s t-test .