BZIP16 Antibody

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Description

Introduction to bZIP16

The bZIP16 transcription factor is a member of the basic region/leucine zipper (bZIP) family in Arabidopsis thaliana. It plays a crucial role in integrating light and hormone signals to regulate seed germination and hypocotyl elongation during early seedling development .

Function of bZIP16

bZIP16 acts as a transcriptional repressor, influencing the expression of genes involved in light, gibberellic acid (GA), and abscisic acid (ABA) signaling pathways. It directly targets and represses genes such as RGL2 and ABA-responsive genes like HVA22B, EM6, AHG1, and STZ, thereby promoting seed germination and hypocotyl elongation .

Binding Activity of bZIP16

bZIP16 specifically binds to the G-box cis-element, a palindromic DNA motif composed of two identical half-sites. This binding activity is crucial for its regulatory functions in gene expression .

Redox Regulation of bZIP16

The activity of bZIP16, like other G-group bZIP transcription factors, can be regulated by environmentally induced changes in the redox state. This regulation affects its DNA binding activity and, consequently, its role in plant responses to environmental cues .

Potential for Antibody Development

While there is no direct information on "BZIP16 Antibody," developing antibodies against bZIP16 could be a valuable tool for studying its function and regulation in plant biology. Such antibodies could facilitate immunoprecipitation, Western blotting, and other techniques to investigate bZIP16's role in plant development and stress responses.

Data Table: Key Features of bZIP16

FeatureDescription
FunctionTranscriptional repressor integrating light and hormone signals
Target GenesRGL2, PIL5, ABA-responsive genes (HVA22B, EM6, AHG1, STZ)
Binding SiteG-box cis-element
RegulationRedox state affects DNA binding activity
Role in Plant DevelopmentPromotes seed germination and hypocotyl elongation

Product Specs

Buffer
Preservative: 0.03% ProClin 300. Constituents: 50% Glycerol, 0.01M PBS, pH 7.4.
Form
Liquid
Lead Time
14-16 weeks (Made-to-order)
Synonyms
BZIP16 antibody; At2g35530bZIP transcription factor 16 antibody; AtbZIP16 antibody
Target Names
BZIP16
Uniprot No.

Target Background

Function
This antibody targets bZIP16, a transcriptional activator that binds to the G-box motif (5'-CACGTG-3') and related cis-acting elements containing the 5'-ACGT-3' core sequence, such as Hex, C-box, and as-1 motifs. It exhibits high binding affinity for the G-box, significantly lower affinity for Hex and C-box, and minimal affinity for the as-1 element. G-box and G-box-like motifs are cis-acting elements found in the promoters of various plant genes regulated by stimuli such as light and hormones. Specifically, bZIP16 binds to the G-box motif (5'-CACGTG-3') within the LHCB2.4 (At3g27690) promoter. It may function as a transcriptional repressor in the light-regulated expression of LHCB2.4. BZIP16 binds DNA as a monomer, and its DNA-binding activity is redox-dependent.
Gene References Into Functions
bZIP16 promotes seed germination and hypocotyl elongation in early Arabidopsis seedling development. [PMID: 23104829](https://www.ncbi.nlm.nih.gov/pubmed/23104829)
Database Links

KEGG: ath:AT2G35530

STRING: 3702.AT2G35530.1

UniGene: At.37663

Protein Families
BZIP family
Subcellular Location
Nucleus.

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