BZIP17 Antibody
Description
BZIP17 Protein Characteristics and Antibody Utility
BZIP17 is a membrane-associated basic leucine zipper (bZIP) transcription factor activated through proteolytic cleavage during stress responses . Antibodies targeting BZIP17 are critical for:
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Detecting proteolytic processing: Western blot analyses reveal cleavage events under salt or ER stress, with full-length (~70 kDa) and processed (~50 kDa) forms observed .
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Subcellular localization tracking: Immunofluorescence and GFP-tagged studies show ER-to-nucleus translocation post-activation .
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Chromatin immunoprecipitation (ChIP): Used to identify genome-wide targets, as demonstrated in heat-stressed MYC-bZIP17 transgenic plants .
Stress Response Regulation
Interaction Networks
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Co-regulation with bZIP28/bZIP60: Shares 132/133 targets with bZIP28 but shows distinct activation kinetics .
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Antagonism with NPR1: BZIP17-mediated UPR suppresses salicylic acid pathways during pathogen responses .
Technical Validation of Antibody Specificity
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Processing assays: Antibodies distinguish between full-length (ER-localized) and cleaved (nuclear) forms .
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Mutant controls: No signal detected in bzip17 knockout lines .
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Tag-based validation: Epitope tags (MYC/GFP) enable antibody cross-verification in transgenic lines .
Research Implications and Limitations
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Functional redundancy: Partial overlap with bZIP28 complicates phenotype interpretation in single mutants .
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Post-translational focus: Transcript levels remain stable under stress, necessitating protein-level antibody assays .
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Species cross-reactivity: Limited data exist for non-Arabidopsis systems, though homologs are implicated in Catharanthus roseus and Boea hygrometrica .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research indicates that the ER-located transcription factors bZIP17 and bZIP60 play a role in salt stress, but this response involves a signaling pathway distinct from the unfolded protein response. PMID: 25704669
- Salt stress induces a signaling cascade involving the processing of AtbZIP17, its translocation to the nucleus, and the upregulation of salt stress genes. PMID: 17662035
- Activated AtbZIP17 enhances salt tolerance under salt stress conditions. PMID: 18721266
