BZIP23 Antibody

Functional Role of BZIP23

BZIP23 belongs to the basic leucine zipper (bZIP) transcription factor family and is critical for:

• Zinc deficiency adaptation: Activates zinc uptake transporters (ZIP1, ZIP4, NAS2) under low zinc conditions .

• Seed vigor regulation: Enhances detoxification pathways (e.g., PER1A activation) to improve rice seed longevity under stress .

• Anthocyanin synthesis: Forms a complex with MdNAC1 in apples to promote pigment accumulation under light .

Applications of BZIP23 Antibody in Research

The antibody enables precise detection and functional characterization of BZIP23 in experiments:

Mechanistic Insights from Antibody-Based Studies

• Zinc sensing: BZIP23 activity is post-translationally regulated by zinc status via a cysteine/histidine-rich N-terminal domain, acting as a zinc sensor .

• Transcriptional activation: Directly binds to PER1A and ZIP4 promoters to upregulate detoxification and zinc uptake genes .

• Crosstalk with ABA signaling: BZIP23-PER1A module interacts with abscisic acid pathways to enhance seed vigor under oxidative stress .

Technical Considerations

• Specificity: Antibodies target conserved regions (e.g., bZIP DNA-binding domain) but may cross-react with homologs like BZIP19 due to 60% sequence similarity .

• Validation: Used in dual-luciferase assays to confirm transcriptional activation of target genes (e.g., PER1A in rice protoplasts) .

Research Implications

• Biofortification: Modulating BZIP23 could improve zinc accumulation in crops .

• Stress tolerance: Overexpression enhances seed longevity and anthocyanin production, valuable for agricultural resilience .