C-Myc (410-419aa) Antibody
Description
Target and Epitope Specificity
The antibody binds to a synthetic peptide corresponding to residues 410–419 of the human p62 c-Myc protein . This sequence is part of the protein’s alpha-helical dimerization domain, making it unsuitable for detecting endogenous c-Myc in most cases . Key features include:
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Cross-reactivity: Specific to human c-Myc; does not react with v-myc or murine c-Myc (except weakly at high concentrations) .
Key Applications
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Western Blot (WB): Detects c-Myc-tagged proteins at dilutions up to 1:30,000 (0.05 µg/mL) .
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Immunoprecipitation (IP): Validated using GenScript’s One-Step IP-Western Kit .
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Immunofluorescence (IF): Demonstrated in transfected 293 cells .
Sensitivity Comparison
Performance Validation
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Western Blot: GenScript’s A00704 outperforms traditional 9E10 clones, showing clear signals at 0.05 µg/mL (1:20,000 dilution) .
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Specificity: No cross-reactivity with v-myc or endogenous murine c-Myc in standard assays .
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Limit of Detection: As low as 20 ng of recombinant protein in chemiluminescent assays .
Research Case Studies
Product Specs
Product Science Overview
c-Myc is a protein encoded by the MYC gene in humans. It is a transcription factor that plays a crucial role in cell cycle progression, apoptosis, and cellular transformation. The c-Myc protein is involved in the regulation of various cellular processes, including cell growth, differentiation, and metabolism. It is known to be overexpressed in many types of cancer, making it a significant target for cancer research and therapy.
The c-Myc protein consists of several domains, each contributing to its function as a transcription factor. The 410-419 amino acid (aa) region of c-Myc is particularly important for its interaction with other proteins and its role in transcriptional regulation. This region is part of the transactivation domain, which is responsible for activating the transcription of target genes.
The Mouse Anti Human c-Myc (410-419aa) antibody is a monoclonal antibody that specifically recognizes the 410-419 amino acid region of the human c-Myc protein. This antibody is produced by immunizing mice with a recombinant human c-Myc protein containing the 410-419 amino acids. The spleen cells from these immunized mice are then fused with myeloma cells to create hybridoma cells that produce the desired antibody .
The Mouse Anti Human c-Myc (410-419aa) antibody is widely used in various research applications, including:
- Western Blotting: This technique is used to detect the presence of the c-Myc protein in cell or tissue samples. The antibody binds to the 410-419 aa region of c-Myc, allowing researchers to visualize and quantify the protein.
- ELISA (Enzyme-Linked Immunosorbent Assay): This method is used to measure the concentration of c-Myc in biological samples. The antibody’s specificity for the 410-419 aa region ensures accurate detection.
- Immunoprecipitation: This technique allows researchers to isolate the c-Myc protein from a complex mixture of proteins. The antibody binds to c-Myc, enabling its purification and subsequent analysis.
- Immunofluorescence: This application involves using the antibody to visualize the localization of c-Myc within cells. The antibody is conjugated to a fluorescent dye, allowing researchers to observe c-Myc distribution under a fluorescence microscope.
The c-Myc protein is a key player in cancer biology due to its role in promoting cell proliferation and inhibiting apoptosis. Overexpression of c-Myc is associated with various cancers, including breast, colon, and lung cancers. The Mouse Anti Human c-Myc (410-419aa) antibody is an essential tool for studying the mechanisms of c-Myc-driven oncogenesis and for developing potential therapeutic strategies targeting c-Myc.
