C19ORF80 Rat
Description
Molecular Structure and Properties
C19ORF80 Rat is a recombinant protein produced in E. coli, consisting of 193 amino acids with a molecular mass of 22.0 kDa . Key structural features include:
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N-Terminal His-tag: Facilitates purification and detection in experimental setups.
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Solubility: Requires 0.1M acetate buffer (pH 4.0) for reconstitution, with limited solubility at higher pH concentrations .
Lipid Metabolism Regulation
C19ORF80 Rat modulates lipid homeostasis through interactions with ANGPTL3 (Angiopoietin-like protein 3). It promotes ANGPTL3 cleavage, enhancing lipoprotein lipase (LPL) activity and reducing triglyceride clearance . This mechanism is critical in regulating serum triglyceride levels.
Autophagy Activation
Thyroid hormone (T3) upregulates C19ORF80 expression, inducing autophagic flux in hepatocytes. Overexpression of C19ORF80 increases lipid droplet (LD) degradation via autolysosome maturation, reducing LD size and triglyceride (TG) content . Key findings include:
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LC3-II Accumulation: Marker of autophagy activation, observed in T3-treated and C19ORF80-overexpressing cells .
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Lipid Droplet Dynamics: C19ORF80 colocalizes with PLIN3 (LD surface protein), enabling LD breakdown .
Pancreatic Beta-Cell Proliferation
C19ORF80 stimulates beta-cell mass expansion, improving glucose tolerance. This effect is mediated by insulin and nutrient intake, positioning it as a potential therapeutic target for diabetes .
Disease Associations
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Obesity: Circulating ANGPTL8 levels are elevated in obesity and reduced post-exercise training . Full-length ANGPTL8 correlates with fasting blood glucose (FBG) in non-obese and obese subjects .
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Genetic Variants: The Arg59Trp polymorphism (rs2278426) in ANGPTL8 is linked to reduced HDL-C and LDL-C levels, suggesting a role in dyslipidemia .
Detection and Quantification
ELISA kits enable precise measurement of C19ORF80 in rat samples:
Experimental and Therapeutic Implications
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Metabolic Disorders: Targeting C19ORF80 may improve insulin sensitivity and lipid profiles in obesity and NAFLD .
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Recombinant Protein Use: Rat C19ORF80 is employed in studies to mimic endogenous ANGPTL8 activity in vitro .
References
Product Specs
Product Science Overview
C19ORF80 is expressed in multiple tissues, with notable expression in adipose tissue, liver, and muscle. Its expression is regulated by various physiological conditions, including fasting and feeding, which suggests its role in metabolic homeostasis.
The primary function of C19ORF80 is to regulate lipid metabolism. It promotes the cleavage of angiopoietin-like protein 3 (ANGPTL3), which in turn regulates serum triglyceride levels . This regulation is crucial for maintaining lipid homeostasis and preventing metabolic disorders such as hypertriglyceridemia.
C19ORF80 exerts its effects by interacting with ANGPTL3, a protein known to inhibit lipoprotein lipase (LPL) activity. By promoting ANGPTL3 cleavage, C19ORF80 indirectly enhances LPL activity, leading to increased triglyceride hydrolysis and reduced serum triglyceride levels.
The expression and activity of C19ORF80 are regulated by various factors, including nutritional status and hormonal signals. For instance, its expression is upregulated during fasting, which aligns with its role in lipid metabolism. Additionally, C19ORF80 is subject to post-translational modifications that modulate its activity and stability.
