At2g29580 Antibody

Introduction to At2g29580 Antibody

The At2g29580 antibody is a specialized immunological tool designed to detect and study the protein encoded by the At2g29580 gene in Arabidopsis thaliana (mouse-ear cress). This gene, also annotated as AtC3H25, encodes a putative CCCH-type zinc finger protein implicated in plant stress responses, particularly iron (Fe) homeostasis . The antibody is utilized in research to investigate protein localization, interaction networks, and regulatory mechanisms under varying physiological conditions.

Gene and Protein Overview

• Gene ID: At2g29580

• UniProt ID: Q9ZW36

• Protein Name: AtC3H25 (CCCH zinc finger protein 25)

• Function: Involved in Fe deficiency responses, potentially regulating transcription or RNA metabolism through zinc finger motifs .

Role in Iron Homeostasis

Studies demonstrate that AtC3H25 interacts with URI (UPSTREAM REGULATOR OF IRT1), a key regulator of Fe uptake in Arabidopsis. Under Fe-deficient conditions, AtC3H25 co-immunoprecipitates with URI-GFP, suggesting its role in modulating Fe deficiency signaling pathways .

Table 1: Peptide Counts of AtC3H25 in Fe-Deficient vs. Fe-Sufficient Conditions

This table highlights the upregulation of AtC3H25 under Fe deficiency, with peptide counts increasing from undetectable levels in Fe-sufficient roots to measurable quantities during Fe stress .

Immunoprecipitation Studies

The At2g29580 antibody has been critical in identifying protein-protein interactions. For example, immunoprecipitation assays using anti-GFP antibodies in pURI:URI-GFP transgenic lines revealed URI-AtC3H25 complexes, linking AtC3H25 to Fe regulation .

Functional Insights

• Genetic Redundancy: AtC3H25 shares partial functional redundancy with other CCCH proteins, complicating phenotypic analysis in single mutants .

• Stress Adaptation: Preliminary data suggest involvement in abiotic stress responses, though further validation is required .

Validation and Specificity Considerations

While widely used, commercial antibodies require rigorous validation. Independent studies on other antibodies (e.g., angiotensin II AT2 receptor antibodies) have shown nonspecific binding and inconsistent results in knockout models . For At2g29580:

• Specificity Tests: No peer-reviewed validation data are publicly available.

• Recommendations: Pair with genetic controls (e.g., At2g29580 mutants) and orthogonal methods (e.g., RNA-seq) to confirm findings.