At2g40140 Antibody

Overview of AT2G40140

AT2G40140, also known as CZF1, SZF2, or ZFAR1, encodes a zinc finger protein with ankyrin-repeat domains. It belongs to the CCCH-type zinc finger family, characterized by cysteine and histidine residues coordinating zinc ions. Key features include:

• Molecular function: RNA binding, nucleic acid metabolism, and stress response regulation .

• Biological role: Mutants exhibit increased susceptibility to pathogens (e.g., Pseudomonas syringae) and reduced salt tolerance, implicating it in abiotic and biotic stress pathways .

AT2G40140 Antibody: Development and Applications

While no commercial antibody specific to AT2G40140 is explicitly detailed in the provided sources, its study likely relies on custom polyclonal antibodies. Such antibodies are critical for:

• Localization studies: Tracking protein expression in plant tissues under stress.

• Western blotting: Validating protein size (~50 kDa predicted) and expression levels in mutants .

• Immunoprecipitation: Identifying interaction partners (e.g., RNA molecules or signaling proteins) .

Functional Insights

• Pathogen resistance: AT2G40140 knockdown lines show compromised defense against bacterial pathogens, suggesting a role in innate immunity .

• Salt tolerance: Mutants display reduced survival under high salinity, linking the protein to ion homeostasis or osmotic stress signaling .

• Gene regulation: The CCCH domain likely mediates post-transcriptional regulation of stress-responsive mRNAs .

Future Research Directions

• Antibody validation: Standardized protocols (e.g., CUT&RUN, IP-MS) could map DNA/RNA binding sites and protein complexes .

• Crop engineering: Orthologs in Brassica napus (AS24 5025 antibody reactivity ) may enable translational studies for stress-resistant crops.