ZFWD3 Antibody

ZFWD3/AtC3H59 Protein Overview

ZFWD3 (AtC3H59) is a non-tandem CCCH zinc finger (non-TZF) protein in Arabidopsis that contains:

• A CCCH zinc finger motif at the N-terminal region.

• A WD40 domain with seven WD40 repeats at the C-terminal region .

This protein is nuclear-localized and plays critical roles in seed germination, seedling development, and seed maturation .

Developmental Roles

Mechanistically, AtC3H59 regulates root meristem cell division and interacts with Desi1, a PPPDE family protein involved in deSUMOylation .

Molecular Interactions

• Interaction Partner: Desi1 (a PPPDE family protein with deSUMOylating activity).

• Interaction Domains:

  • AtC3H59 binds Desi1 via its WD40 domain.

  • Desi1 interacts through its C-terminal region .

This interaction suggests a role in post-translational modification pathways, potentially regulating developmental processes through SUMOylation .

Evolutionary Conservation

ZFWD3/AtC3H59 orthologs are highly conserved in:

• Arabidopsis lyrata

• Camelina sativa

• Brassica rapa

Sequence alignment shows strong conservation in the CCCH zinc finger and WD40 domains .

Antibody-Related Context

While the search results do not describe a specific "ZFWD3 Antibody," general antibody mechanisms and applications are well-documented:

• Antibodies are Y-shaped proteins that bind antigens with high specificity .

• Neutralizing antibodies (e.g., bimekizumab , ADG-2 ) block pathogenic proteins, a strategy that could theoretically apply to plant proteins like ZFWD3 if targeted.

Research Gaps and Opportunities

No studies in the provided sources describe the development or use of antibodies targeting ZFWD3/AtC3H59. Future research could explore:

• Antibody Generation: For detecting ZFWD3 in plant tissues.

• Functional Blocking: To study ZFWD3’s role in development.

• Agricultural Applications: Modulating seed traits via ZFWD3 interaction pathways.