CYP734A1 Antibody

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Description

Introduction

The CYP734A1 antibody is a research tool used to detect and study the Cytochrome P450, Family 7, Subfamily A, Polypeptide 1 (CYP7A1) protein . CYP7A1 is an enzyme involved in various metabolic processes . Antibodies that target CYP7A1 are valuable in various scientific applications, including protein detection via Western blotting, ELISA, immunofluorescence, and immunohistochemistry .

Characteristics of CYP7A1

CYP7A1, or Cytochrome P450, Family 7, Subfamily A, Polypeptide 1, is a member of the cytochrome P450 monooxygenase superfamily, which are enzymes that play a crucial role in the metabolism of various endogenous and exogenous compounds .

CYP734A1 Antibody Details

FeatureDescription
TargetCYP7A1 (Cytochrome P450, Family 7, Subfamily A, Polypeptide 1)
Binding SpecificityAA 351-400
ReactivityHuman, Mouse, and Rat
HostRabbit
ClonalityPolyclonal
ApplicationsWestern Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
PurificationPurified by Protein A
ImmunogenKLH conjugated synthetic peptide derived from human CYP7A1
IsotypeIgG

Applications in Research

The CYP734A1 antibody is utilized across a range of research applications to investigate the role and function of CYP7A1 .

  • Western Blotting (WB): This technique is commonly used to detect the presence and size of the CYP7A1 protein in cell lysates or tissue samples .

  • ELISA: The antibody can be used in ELISA assays to quantify CYP7A1 protein levels in various samples .

  • Immunofluorescence (IF): This method allows for the visualization of CYP7A1 protein expression and localization within cells .

  • Immunohistochemistry (IHC): Researchers use the antibody to detect CYP7A1 protein in tissue sections, providing insights into its expression patterns in different tissues and disease states .

Related Research

CYP734A1 and CYP72C1 are involved in steroid-mediated signal transduction in Arabidopsis thaliana . Genetic analysis has shown that these enzymes affect plant growth throughout development . While the CYP734A subfamily inactivates brassinosteroids through C-26 hydroxylation, the biochemical activity of CYP72C1 is not known .

Product Specs

Buffer
Preservative: 0.03% ProClin 300. Constituents: 50% Glycerol, 0.01M PBS, pH 7.4.
Form
Liquid
Lead Time
14-16 weeks (made-to-order)
Synonyms
CYP734A1; BAS1; At2g26710; F18A8.8; Cytochrome P450 734A1; Protein PHYB ACTIVATION-TAGGED SUPPRESSOR 1
Target Names
CYP734A1
Uniprot No.

Target Background

Function
CYP734A1 is a cytochrome P450 enzyme involved in the inactivation and homeostasis of brassinosteroids (BRs). Specifically, it inactivates the BRs castasterone and brassinolide via carbon 26 hydroxylation. This enzyme functions in concert with CYP72C1 to regulate BR levels and modulate photomorphogenesis.
Database Links

KEGG: ath:AT2G26710

STRING: 3702.AT2G26710.1

UniGene: At.43115

Protein Families
Cytochrome P450 family
Subcellular Location
Membrane; Single-pass membrane protein.

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