The CYP734A1 antibody is a research tool used to detect and study the Cytochrome P450, Family 7, Subfamily A, Polypeptide 1 (CYP7A1) protein . CYP7A1 is an enzyme involved in various metabolic processes . Antibodies that target CYP7A1 are valuable in various scientific applications, including protein detection via Western blotting, ELISA, immunofluorescence, and immunohistochemistry .
CYP7A1, or Cytochrome P450, Family 7, Subfamily A, Polypeptide 1, is a member of the cytochrome P450 monooxygenase superfamily, which are enzymes that play a crucial role in the metabolism of various endogenous and exogenous compounds .
The CYP734A1 antibody is utilized across a range of research applications to investigate the role and function of CYP7A1 .
Western Blotting (WB): This technique is commonly used to detect the presence and size of the CYP7A1 protein in cell lysates or tissue samples .
ELISA: The antibody can be used in ELISA assays to quantify CYP7A1 protein levels in various samples .
Immunofluorescence (IF): This method allows for the visualization of CYP7A1 protein expression and localization within cells .
Immunohistochemistry (IHC): Researchers use the antibody to detect CYP7A1 protein in tissue sections, providing insights into its expression patterns in different tissues and disease states .
CYP734A1 and CYP72C1 are involved in steroid-mediated signal transduction in Arabidopsis thaliana . Genetic analysis has shown that these enzymes affect plant growth throughout development . While the CYP734A subfamily inactivates brassinosteroids through C-26 hydroxylation, the biochemical activity of CYP72C1 is not known .