CYP76C2 Antibody

CYP76C2 Protein Overview

CYP76C2 (Cytochrome P450 76C2) is encoded by the CYP76C2 gene (TAIR: At3g26280) and belongs to a small multigene family with four members . Cytochrome P450 enzymes are heme-containing monooxygenases involved in detoxification, hormone biosynthesis, and secondary metabolism.

Antibody Development and Applications

The CYP76C2 antibody was generated using a cDNA clone (B72) isolated during studies of the hypersensitive response (HR) triggered by bacterial pathogens like Pseudomonas syringae . Key applications include:

• Localization studies: Tracking CYP76C2 expression during HR, leaf senescence, and wounding.

• Functional assays: Investigating its role in PCD pathways induced by pathogens or abiotic stressors (e.g., lead nitrate) .

• Western blotting: Detecting CYP76C2 in protein extracts from stressed tissues.

Expression Patterns

CYP76C2 expression is upregulated under conditions associated with cell death:

Key Findings

• HR and pathogen defense: CYP76C2 transcription increases during incompatible plant-pathogen interactions, suggesting a role in amplifying oxidative bursts or lipid peroxidation during HR .

• Senescence and aging: Elevated expression in senescing leaves and aging cell cultures links CYP76C2 to developmental PCD .

• Abiotic stress: Lead nitrate-induced necrosis correlates with CYP76C2 upregulation, implicating it in heavy metal toxicity responses .

Technical Considerations

• Cross-reactivity: No cross-reactivity reported in mutants (e.g., cyp76c2 T-DNA lines), validating specificity .

• Limitations: No commercial sources are documented; most studies use custom-made antibodies .

Future Directions

Further research should address:

• Structural characterization of CYP76C2.

• Interaction partners in PCD pathways.

• Engineering transgenic plants with modulated CYP76C2 activity to explore agricultural applications.