CYP81F2 Antibody
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
CYP81F2 is involved in the biosynthesis of indole glucosinolates. This enzyme catalyzes hydroxylation reactions within the indole ring of glucosinolates. Specifically, it converts indol-3-yl-methylglucosinolate (I3M) to 4-hydroxy-indol-3-yl-methylglucosinolate (4OH-I3M) and/or 1-hydroxy-indol-3-yl-methylglucosinolate (1OH-I3M). These hydroxylated intermediates are further processed by indole glucosinolate methyltransferases 1 and 2 (IGMT1 and IGMT2) to form 4-methoxy-indol-3-yl-methylglucosinolate (4MO-I3M) and 1-methoxy-indol-3-yl-methylglucosinolate (1MO-I3M). CYP81F2 contributes to plant defense mechanisms against the green peach aphid (Myzus persicae), a generalist phloem-feeding herbivore. Furthermore, it plays a crucial role in the biosynthesis of antifungal indole glucosinolate metabolites. Its function is essential for pathogen-induced accumulation of 4MO-I3M, which is subsequently activated by the atypical myrosinase BGLU26/PEN2. CYP81F2 is required for the biosynthesis of tryptophan-derived antifungal compounds and contributes to non-host resistance against the necrotrophic fungal pathogen Plectosphaerella cucumerina and the non-adapted fungal pathogen Colletotrichum gloeosporioides.
One study identified CYP81F2 as the gene encoding Indole Glucosinolate Modifier 1 (IGM1), a metabolic quantitative trait locus (QTL) influencing the accumulation of two modified indole glucosinolates. This study also demonstrated that CYP81F2 contributes to defense against the green peach aphid. PMID: 19293369
