CYP85A2 Antibody
Description
Role of CYP85A2 in Plants
CYP85A2 is a cytochrome P450 enzyme involved in the final step of brassinosteroid biosynthesis, which is essential for plant growth and development . Brassinosteroids are plant hormones that regulate cell elongation, cell division, and vascular differentiation. The enzyme's activity is crucial for maintaining normal plant morphology and responding to environmental stresses.
Importance of Antibodies in Research
Antibodies are essential tools in biological research for detecting and quantifying proteins. An antibody specific to CYP85A2 would allow researchers to study its expression levels, subcellular localization, and interactions with other proteins. This could provide insights into how brassinosteroid biosynthesis is regulated and how it impacts plant physiology.
Potential Applications of a CYP85A2 Antibody
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Western Blotting and Immunoprecipitation: These techniques would enable researchers to quantify CYP85A2 protein levels and study its interactions with other proteins involved in brassinosteroid biosynthesis.
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Immunofluorescence Microscopy: This method would allow for the visualization of CYP85A2's subcellular localization, which is crucial for understanding its function in plant cells.
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ELISA (Enzyme-Linked Immunosorbent Assay): This technique could be used to quantify CYP85A2 levels in plant extracts, providing insights into how its expression changes under different conditions.
Research Findings and Data
While specific data on a CYP85A2 antibody is not available, research on CYP85A2 itself highlights its importance in plant biology. For example, mutations in CYP85A2 lead to reduced brassinolide accumulation, increased sensitivity to abscisic acid, and enhanced drought tolerance .
Table: Effects of CYP85A2 Mutations
| Phenotype | Description |
|---|---|
| Reduced Brassinolide Accumulation | Impaired brassinosteroid biosynthesis |
| Increased Sensitivity to ABA | Enhanced response to abscisic acid |
| Enhanced Drought Tolerance | Improved survival under drought conditions |
| Morphological Changes | Round, darker green leaves with short petioles |
References PMC: Farnesylation-mediated subcellular localization is required for CYP85A2 function in Arabidopsis. NPH: HBI1 acts downstream of ERECTA and SWR1 in regulating plant development. Frontiers in Pharmacology: Development of an IgY Antibody-Based Immunoassay for the Screening of the CYP2E1 Inhibitor/Enhancer from Herbal Medicines. Semantics Scholar: Arabidopsis CYP85A2, a Cytochrome P450, Mediates the Baeyer-Villiger Oxidation of Castasterone to Brassinolide in Brassinosteroid Biosynthesis. Plant Physiology: Genetic Evidence for the Reduction of Brassinosteroid Levels by a BAHD Acyltransferase. PharmGKB: Cytochrome P450, family 2, subfamily C, polypeptide 8. TAIR: Locus AT3G30180 (BR6OX2). Taylor & Francis: Endogenous level of abscisic acid down-regulated by brassinosteroids in Arabidopsis.
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
This antibody targets CYP85A2, a cytochrome P450 enzyme that catalyzes the C6-oxidation step in brassinosteroid biosynthesis. Specifically, it converts 6-deoxocastasterone to castasterone, and castasterone to brassinolide. Additional substrates include 6-deoxoteasterone (converted to teasterone), 3-dehydro-6-deoxoteasterone (converted to 3-dehydroteasterone), and 6-deoxotyphasterol (converted to typhasterol).
Key Findings Regarding CYP85A2 Function:
- CYP85A2's function in Arabidopsis requires farnesylation. Loss of either CYP85A2 or its farnesylation leads to decreased brassinolide accumulation, increased sensitivity to abscisic acid (ABA), and enhanced drought tolerance. (PMID: 27455172)
- seu cyp85A2 double mutants exhibit significant disruptions in gynoecial and ovule development. (PMID: 20836864)
- CYP85A1 and CYP85A2 promoters display peak activity during the first week post-germination, primarily within vascular tissues. (PMID: 15821873)
- CYP85A2 functions as a bifunctional enzyme, exhibiting both BR C-6 oxidase (C-6 oxidation of brassinosteroids) and brassinolide (BL) synthase activity. This cytochrome P450 mediates Baeyer-Villiger oxidation in plants. (PMID: 16024588)
- CYP85A2 catalyzes the lactonization of not only castasterone but also teasterone and typhasterol. (PMID: 18685225)
