CANT1 Antibody
Description
Overview of CANT1 Antibody
CANT1 (Calcium-Activated Nucleotidase 1) antibodies are immunological tools designed to detect and study the CANT1 protein, a member of the apyrase family localized in the endoplasmic reticulum (ER) and Golgi apparatus. These antibodies enable researchers to investigate CANT1’s roles in proteoglycan biosynthesis, glycosylation, and skeletal development, as well as its implications in diseases such as Desbuquois dysplasia type 1 (DBQD1) and cancer .
Proteoglycan Biosynthesis and Skeletal Disorders
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Role in DBQD1: Knock-out mouse models revealed CANT1’s critical role in glycosaminoglycan (GAG) chain synthesis and sulfation. Loss of CANT1 causes ER enlargement, delayed proteoglycan secretion, and growth plate defects, recapitulating DBQD1 phenotypes .
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Mechanism: CANT1 hydrolyzes UDP, a byproduct of glycosyltransferase reactions, to regulate nucleotide levels in the ER/Golgi .
Cancer Research
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Hepatocellular Carcinoma (HCC): CANT1 overexpression correlates with poor prognosis, advanced tumor stage, and immune cell infiltration (e.g., CD8+ T cells). It serves as an independent prognostic marker (AUC = 0.938) .
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Retinoblastoma (RB): LncRNA CANT1 suppresses tumor progression by inhibiting PI3Kγ/Akt signaling. Overexpression reduces tumor growth in vitro and in vivo .
Technical Validation
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Western Blot: Detected in LNCaP (prostate cancer), U2OS (osteosarcoma), and A549 (lung cancer) cell lines .
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Subcellular Localization: Perinuclear staining in PC-3 prostate cancer cells .
Disease Associations
Therapeutic Potential
Recommended Workflows
| Application | Dilution | Sample Preparation |
|---|---|---|
| Western Blot | 1:500–1:5000 | Reduce lysates with β-mercaptoethanol |
| ICC/IF | 10 µg/mL | Fix cells with paraformaldehyde |
Troubleshooting
Product Specs
Target Background
- CANT1 long non-coding RNA demonstrates therapeutic efficacy by correcting aberrant long non-coding cascade in malignant uveal melanoma. PMID: 28330694
- The Multiple Epiphyseal Dysplasia (MED) phenotype is allelic to the more severe Desbuquois dysplasia phenotype, identifying CANT1 as a second locus for recessively inherited MED. PMID: 28742282
- A novel mutation in CANT1, c.467C>T (p.Ser156Phe), has been identified in three Indian patients with Desbuquois dysplasia, Kim type, from two families. PMID: 25486376
- Studies on proteoglycan synthesis in CANT1 mutated patient fibroblasts revealed significantly reduced GAG synthesis in the presence of beta-D-xyloside, suggesting a role for CANT1 in proteoglycan metabolism. PMID: 22539336
- Novel mutations in the CANT1 gene have been reported in three cases of Desbuquois dysplasia type I and fetal hydrops. PMID: 21654728
- The age of the founder mutation has been estimated to be approximately 1420 years. PMID: 21412251
- CANT1 is commonly overexpressed in a majority of primary prostate carcinomas and in the precursor lesion PIN, potentially serving as a novel prognostic biomarker. PMID: 21435463
- The clinical-radiographic spectrum produced by CANT1 mutations has been expanded to include Desbuquois dysplasia type 2 and Kim variant. PMID: 21037275
- This research involved the cloning, expression, and characterization of a human enzyme belonging to a new family of extracellular nucleotidases. PMID: 12234496
- The soluble apyrase is a calcium-binding protein, as evidenced by saturable Ca2+-dependent changes in intrinsic tryptophan fluorescence, UV difference absorption spectra, and Ca2+-triggered transition from enzymatically inactive form to active enzyme. PMID: 12600208
- The importance of the dimeric state for enzymatic activity and biological function in this nucleotidase was investigated by mutating isoleucine 170. PMID: 18067325
- Human soluble calcium-activated nucleotidase inhibits coagulation in vitro and thrombosis in vivo. PMID: 18222531
- The two novel ETV4 fusion partners share predominant common characteristics: androgen-induction and prostate-specific expression. PMID: 18451133
- Mutations in CANT1 have been identified as a cause of Desbuquois dysplasia. PMID: 19853239
