At2g01600 Antibody

Gene and Protein Identification

AT2G01600 is a locus in the Arabidopsis thaliana genome encoding an ENTH/ANTH/VHS superfamily protein. Key identifiers include:

This protein is distinct from antibodies but interacts with clathrin and SNARE proteins during vesicle formation.

Functional Role in Clathrin-Mediated Endocytosis

The AT2G01600-encoded protein (hereafter At2g01600) facilitates vesicle trafficking by acting as a clathrin adaptor. Key findings include:

Mechanistic Insights

• Clathrin interaction: At2g01600 binds clathrin heavy chains via its C-terminal domain, enabling clathrin-coated vesicle (CCV) formation during cytokinesis and membrane recycling .

• VAMP72 interaction: It directly interacts with the SNARE domain of VAMP72, a longin-type R-SNARE involved in membrane fusion .

• Membrane lipid binding: The N-ANTH domain preferentially binds phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P₃), a key regulator of membrane trafficking .

Experimental Evidence

Domain Architecture

At2g01600 contains an N-terminal N-ANTH domain, distinct from canonical ANTH domains due to:

• Phosphoinositide specificity: Higher affinity for PtdIns(3,4,5)P₃ vs. PtdIns(4,5)P₂ .

• Vesicle tubulation: Induces membrane tubulation, a hallmark of adaptor proteins in endocytosis .

Protein Interactions

Localization and Expression Patterns

• Cellular localization:

  • Dividing cells: Accumulates at expanding cell plates during cytokinesis .

  • Non-dividing cells: Detectable at plasma membranes and endosomes .

• Promoter-driven expression: Native promoter-driven GFP fusions replicate endogenous localization patterns .

Functional Studies

• Cytokinesis: Disruption of At2g01600 impairs cell plate formation, highlighting its role in plant cell division .

• Membrane recycling: Required for recycling of R-SNARE proteins, ensuring vesicle fusion efficiency .

Comparative Analysis