CAPRIN2 Antibody, FITC conjugated
Description
Definition and Target
CAPRIN2 (Caprin Family Member 2) is a 1127-amino-acid protein involved in Wnt/β-catenin signaling, cell cycle regulation, and RNA granule formation . The FITC-conjugated antibody targets specific epitopes (e.g., residues 101–200 or 83–100) within this protein, enabling fluorescent detection in assays like immunofluorescence (IF) or immunohistochemistry (IHC) .
Key Attributes of CAPRIN2 Antibody, FITC Conjugated
| Parameter | Details |
|---|---|
| Host Species | Rabbit (polyclonal) |
| Conjugate | FITC (Fluorescein Isothiocyanate) |
| Immunogen | KLH-conjugated synthetic peptide (human CAPRIN2 residues 101–200) |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Sheep, Pig, Horse, Chicken |
| Applications | Western Blot (WB), IF (IHC-P/IHC-F/ICC) |
| Dilution Range | WB: 1:300–1:5000; IF/IHC: 1:50–1:200 |
| Concentration | 1 µg/µL |
| Storage | -20°C (avoid freeze-thaw cycles) |
| Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.03% ProClin300, 50% Glycerol |
| Gene ID | 65981 |
| UniProt ID | Q6IMN6 |
Functional Insights
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Wnt Signaling Modulation: CAPRIN2 stabilizes cytosolic β-catenin and enhances LEF-1-dependent transcriptional activity, promoting Wnt target gene expression .
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Cell Cycle Regulation: Acts as a proapoptotic inhibitor, with roles in neuronal and splenic tissues .
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Subcellular Localization: Detected in cytoplasm, nucleoplasm, and centrosomes .
Experimental Validation
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Immunofluorescence: Strong cytoplasmic and nuclear staining in human hippocampal neurons .
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Western Blot: Detects endogenous CAPRIN2 in human, mouse, and rat lysates .
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Cross-Reactivity: Confirmed reactivity in sheep, pig, and horse tissues .
Research Validation
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research has unveiled a previously unknown role of CAPRIN2 in facilitating LRP5/6 constitutive phosphorylation during G2/M phase. This occurs through the formation of a quaternary complex with CDK14, Cyclin Y, and LRP5/6. PMID: 27821587
- The C1q-related domain of CAPRIN2 forms a flexible homotrimer, mediated by calcium. This trimeric assembly is crucial for CAPRIN2's functionality. PMID: 25331957
- The regulated expression of EEG-1 is involved in the coordinated regulation of growth observed as erythroblasts transition from a highly proliferative state to their terminal differentiation phase. PMID: 14593112
- CAPRIN2 promotes activation of the canonical Wnt signaling pathway by regulating LRP5/6 phosphorylation. PMID: 18762581
