CAR10 Antibody

CAR10 Antibodies Targeting ADAM12 (ADAM Metallopeptidase Domain 12)

CAR10 (ADAM12) is a 909-amino acid transmembrane protein encoded by the ADAM12 gene. It plays roles in skeletal muscle regeneration, cell fusion, and extracellular matrix remodeling, with elevated expression in placenta, testis, and cancers like lung adenocarcinoma .

Key Applications of Anti-CAR10 (ADAM12) Antibodies

Research Findings:

• ADAM12/CAR10 promotes tumor metastasis in lung adenocarcinoma by regulating EMT via miR-30/203 and SNAI1/2 .

• Knockdown of CAR10 suppresses invasion and migration in A549 and PC9 lung cancer cells, correlating with improved prognosis .

Anti-CAR Linker Antibodies (G4S/Whitlow Sequences)

These antibodies target conserved linker sequences (e.g., G4S or Whitlow/218) in single-chain variable fragment (scFv)-based CAR constructs, enabling universal detection of CAR-T cells irrespective of their antigen specificity .

Workflow Applications in CAR-T Research

Advantages Over Anti-Idiotype Antibodies:

• Broad utility: Detect any scFv-based CAR construct with G4S/Whitlow linkers.

• Time-efficient: Avoid custom reagent development for each CAR variant.

Recent Use Cases:

• Diagnostic validation: Monitoring CAR-T persistence in hematologic malignancies .

• Functional studies: Linking CAR expression levels to cytotoxic activity .

Comparative Analysis of CAR10 Antibody Types

Future Directions

• ADAM12-targeting antibodies: Potential therapeutic applications in metastatic cancers via EMT pathway inhibition .

• Anti-linker antibodies: Critical for standardizing CAR-T manufacturing and improving clinical safety .