CASP12 Antibody
Description
Structure and Function of the CASP12 Antibody
The CASP12 Antibody is primarily produced as a polyclonal antibody, generated by immunizing rabbits with synthetic peptides derived from mouse Caspase 12 (catalog #bs-1105R) . Its specificity targets regions spanning amino acids 201–300/420, ensuring broad recognition of Caspase 12 isoforms. The antibody is purified via Protein A chromatography and formulated in a TBS buffer with stabilizers for long-term storage . Key specifications include reactivity with mouse and rat tissues, suitability for Western blot (WB), ELISA, immunohistochemistry (IHC), and flow cytometry (FCM) .
| Parameter | Details |
|---|---|
| Host | Rabbit |
| Immunogen | KLH-conjugated synthetic peptide |
| Reactivity | Mouse, Rat |
| Applications | WB, ELISA, FCM, IHC-P, IHC-F, IF |
| Concentration | 1 µg/µL |
| Storage | TBS buffer with 50% glycerol |
Applications in Research and Diagnostics
The CASP12 Antibody has emerged as a critical tool in studying Caspase 12’s roles in inflammation, apoptosis, and cancer progression. Key applications include:
a. Cancer Prognostics
In cervical cancer (CC), elevated CASP12 expression correlates with improved 3-year survival rates (88.2% vs. 63.2% in low-expression groups) and serves as a diagnostic biomarker with an area under the ROC curve (AUC) of 0.865 . The antibody enables quantitative detection via qRT-PCR, revealing significant downregulation in CC tissues compared to controls (P < 0.05) .
b. Apoptosis Mechanisms
In renal tubular cells, the antibody has demonstrated Caspase 12’s activation during cisplatin-induced apoptosis, where it cleaves procaspase 12 prior to caspases 3 and 9 . This pathway involves oxidative stress from ER cytochrome P450 interactions, a mechanism validated by antibody-mediated inhibition of apoptosis in transfected cells .
c. Inflammation and Invasion
In nasopharyngeal carcinoma (NPC), the antibody identified Caspase 12’s role in NF-κB activation via IκBα degradation, enhancing matrix metalloproteinase-9 (MMP-9) expression and tumor invasion . siRNA knockdown or Casp12 inhibitors reduced invasion by ~23–25%, confirming its functional relevance .
Clinical Significance and Population Genetics
a. Sepsis Susceptibility
The CASP12 gene exhibits a polymorphism generating either a functional (Csp12L) or inactive (Csp12S) protein. Populations of African descent predominantly carry the functional variant, associated with heightened bacterial responses (e.g., to LPS) and increased sepsis risk . The antibody facilitates population-wide screening for this polymorphism, aiding personalized medicine approaches .
b. Evolutionary Insights
The truncated Csp12S allele became fixed in non-African populations ~60–100 thousand years ago, likely conferring sepsis resistance in high-density populations . The antibody’s ability to distinguish isoforms supports studies linking this adaptation to ancient disease pressures .
Product Specs
Target Background
- Piezo1 plays a significant role in the apoptosis of human osteoarthritis-derived chondrocytes through a caspase-12-dependent pathway. PMID: 28731145
- A study indicated the potential effect of Casp12 on the activation of NF-kappaB by the degradation of IkappaBalpha in NPC cells. PMID: 28380444
- The CASP12 genotype does not influence the phenotype of systemic lupus erythematosus in African-Americans. PMID: 26973091
- Endoplasmic reticulum stress may be associated with apoptosis of LECs, resulting in cataract formation in diabetic patients. PMID: 27130368
- Endoplasmic reticulum stress related factor ATF6 and caspase-12 trigger apoptosis in neonatal hypoxic-ischemic encephalopathy. PMID: 26261584
- Expression of caspase-12 was linked to decreased systemic and adipose tissue inflammation in a cohort of African American obese children. PMID: 26582949
- RNA interference-induced Caspase-12 silencing increased NOD1, hBD1, and hBD2 expression. PMID: 25503380
- The functional CASP12L allele is not a marker for susceptibility and/or severity of CAP. PMID: 24586588
- EtOH-induced activation of caspase-12 could be one of the underlying mechanisms of hepatocyte apoptosis. PMID: 24224909
- Distribution of CASP12 alleles (#rs497116) in African-Americans (AA) with rheumatoid arthritis (RA). There was no difference in the overall distribution of genotypes within AA with RA, but homozygous patients had lower baseline joint-narrowing scores. PMID: 24515649
- CASPASE-12 does not influence the susceptibility to Candida sepsis, nor does it have any effect on the serum cytokine concentrations in Candida sepsis patients during the course of infection. PMID: 21706251
- To examine the role of CASP12 in down-regulating inflammation, it is hypothesized that pathogens which exploit the inflammatory response are restrained by an active CASP12 gene product. PMID: 21872999
- Caspase 12 has acquired deleterious mutations. PMID: 12054529
- Pathways involving this enzyme are involved in a neurodegenerative disease in vivo, and thus offer novel potential targets for the treatment of prion disorders. PMID: 14532116
- Na/Ca exchanger overexpression activates caspase-12. PMID: 15033764
- Csp12-L attenuates the inflammatory and innate immune response to endotoxins and may constitute a risk factor for developing sepsis. PMID: 15129283
- Observational study of genotype prevalence. (HuGE Navigator) PMID: 16917906
- The activation of caspase-9 and depolarization of mitochondrial membrane potential were induced by BIK, which were decreased concomitant with caspase-12 silenced. PMID: 17574210
- The proteolytic activity of caspase-12 is confined to its own proenzyme. PMID: 18332441
- hRPE cells express a high level of caspase-12S. The regulated expression of caspase-12S suggests that this caspase recruitment domain (CARD)-only protein may be an endogenous dominant negative regulator that modulates inflammatory responses in hRPE cells. PMID: 18791174
- Estrogen-mediated inhibition of Csp-12L expression is a built-in mechanism that has evolved to protect females from Listeria monocytogenes infection. PMID: 19447924
- The NOD2/RIP2 pathway has a role in recognition of Yersinia, but caspase-12 does not modulate innate host defense against Y. pestis. PMID: 19721713
- The calcium-calpain-caspase-12-caspase-3 cascade is altered in F508del-CFTR expressing cells. PMID: 20041182
- Reactive oxygen species promote caspase-12 expression and tubular apoptosis in diabetic nephropathy. PMID: 20299359
- Observational study of gene-disease association. (HuGE Navigator) PMID: 20299368
- Observational study of gene-disease association. (HuGE Navigator) PMID: 20423816
- Observational study of gene-disease association. (HuGE Navigator) PMID: 20577092
- Constitutively active forms of caspase-12 (rev-Deltapro1 and rev-Deltapro2) could induce cell death in cells transfected with the corresponding expression vectors. PMID: 20646990
Customer Reviews
Applications : Western blot assays
Sample type: Human cells
Review: Cleavage of caspase 12 was detected by Western blot. Combined treatment with tBHP and HDACi induced apoptosis in HCC cells. Apoptosis of HCC cells after treatment with tBHP (25 μmol/L), SAHA (1 mol/L), MS275 (1 μmol/L), or combinatorial treatment with tBHP and HDACi.
