CAV1 Recombinant Monoclonal Antibody
Shipped with Ice Packs 
In Stock 
Description
Definition and Target Specificity
CAV1 Recombinant Monoclonal Antibody is a laboratory-engineered immunoglobulin produced using recombinant DNA technology. It specifically binds to human caveolin-1 (UniProt ID: Q03135), a 22 kDa protein encoded by the CAV1 gene (Entrez Gene ID: 857). This antibody reacts with human, mouse, and rat CAV1 isoforms, including both α and β forms, which are implicated in cancer metastasis and cellular signaling pathways .
Development and Epitope Mapping
Monoclonal antibodies against CAV1 have been generated using advanced methods, including:
-
Cav-1 knockout mice to preserve self-tolerance and enhance specificity .
-
Epitope-focused immunization targeting domains such as the N-terminus (amino acids 1–31), scaffolding domain (80–101), and C-terminal regions .
Key antibody groups identified:
| Group | Binding Region | Number of Antibodies | Affinity (Kd) |
|---|---|---|---|
| N1-31 | N-terminus (1–31) | 5 | – M |
| N32-80 | N-terminus (32–80) | 3 | – M |
| CSD | Scaffolding Domain (80–101) | 2 | – M |
| Cav-1-C | C-terminal half | 1 | M |
These antibodies recognize six distinct epitopes, enabling precise targeting of secreted CAV1 in cancer microenvironments .
Reactivity and Applications
Preclinical Efficacy
-
In Prostate Cancer Models: Anti-CAV1 antibodies reduced tumor cell viability by 60–80% in DU145 and LNCaP cell lines .
-
Co-Immunoprecipitation Data: Both α and β CAV1 isoforms form multimeric complexes in cancer cell secretions, which are effectively neutralized by N1-31 and N32-80 group antibodies .
Clinical Relevance
-
Serum Biomarker: Elevated CAV1 levels in patient serum predict poor prognosis .
-
Therapeutic Potential: Humanized monoclonal antibodies are under development for clinical trials .
Ongoing Research and Limitations
Product Specs
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Description
CUSABIO's recombinant monoclonal antibody targeting CAV1 is generated through a robust process. Immunization of a rabbit with a synthesized peptide from human CAV1 initiates the process. B cells are isolated from the immunized rabbit, and RNA is extracted from these cells. This extracted RNA is then reverse-transcribed into cDNA, which serves as a template for extending CAV1 antibody genes using degenerate primers. These extended genes are introduced into a plasmid vector and transfected into host cells for expression. The CAV1 recombinant monoclonal antibody is purified from the cell culture supernatant through affinity chromatography and subjected to ELISA, WB, and FC applications. It exhibits specific reactivity with human CAV1 protein.
CAV1 is a structural protein that forms oligomers, creating caveolin complexes. These complexes are crucial for shaping and stabilizing caveolae on the cell membrane. Caveolae and CAV1 play significant roles in lipid transport and metabolism, particularly in regulating lipid droplet formation, lipid uptake, and cholesterol trafficking. CAV1 has been linked to various cellular responses to stress, including oxidative stress, mechanical stress, and cellular damage, potentially playing a protective role in some instances.
Form
Liquid
Lead Time
Typically, we are able to dispatch products within 1-3 business days after receiving your order. Delivery time may vary depending on the chosen purchasing method or location. For specific delivery timeframes, please consult your local distributors.
Synonyms
Caveolin-1, CAV1, CAV
Target Names
Uniprot No.
Target Background
Function
CAV1 acts as a scaffolding protein within caveolar membranes. It forms a stable heterooligomeric complex with CAV2, targeting lipid rafts and driving caveolae formation. CAV1 facilitates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to caveolae. It interacts directly with G-protein alpha subunits, potentially regulating their activity. CAV1 participates in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. CAV1 recruits CTNNB1 to caveolar membranes, potentially regulating CTNNB1-mediated signaling through the Wnt pathway. Additionally, CAV1 negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts, leading to its subsequent degradation.
Gene References Into Functions
- Using proteomics profiling, a new protein, caveolin-1, was identified. This protein is significantly and consistently augmented in CLL cells after these lymphocytes interact with stromal cell lines. PMID: 28971726
- Research demonstrates that ITGB1-dependent upregulation of caveolin-1 (CAV1) switches TGFbeta signaling from tumor-suppressive to oncogenic in prostate cancer. This study suggests that TGFbeta signaling and beta1 integrins are potential therapeutic targets in prostate cancer over-expressing CAV1, contributing to a better understanding of TGFbeta's dual role in tumor biology. PMID: 29402961
- Results indicate that CAV-1 can promote anchorage-independent growth and anoikis resistance in detached SGC-7901 cells. This is linked to the activation of Src-dependent epidermal growth factor receptor-integrin beta signaling, as well as the phosphorylation of PI3K/Akt and MEK/ERK signaling pathways. PMID: 30088837
- Cav-1 expression is up-regulated in endothelial cells of atherosclerotic lesions. PMID: 29746866
- Sinonasal inverted papillomas lesions show increased Caveolin-1 immunopositivity compared to nasal polyposis. Additionally, smokers exhibit significantly increased immunopositivity. PMID: 30297114
- Findings suggest that weak stromal CAV1 expression in CRLM is an adverse prognostic factor in patients undergoing liver resection for liver-only colorectal metastases. PMID: 28515480
- This research provides evidence that KIF13B and NPHP4 are both required for the establishment of a specialized caveolin-1 membrane microdomain at the ciliary transition zone. This is essential for Shh-induced accumulation of SMO in the primary cilium and for the activation of GLI-mediated target gene expression. PMID: 28134340
- High CAV1 expression is associated with gastric cancer cell migration. PMID: 30015970
- Research shows that progression-related loss of stromal caveolin 1 levels promotes the growth of human PC3 xenografts and mediates radiation resistance. PMID: 28112237
- Results indicate a decreasing trend of cav-1 (transcripts I and II) in tumoral tissues, particularly in stages I and II. This seems to be associated with the incidence and promotion of breast cancer, especially in the initial stages. PMID: 28857238
- Minor alleles for SNPs rs3779512, rs7804372, and rs1049337 may be associated with a higher risk of hypertriglyceridemia. PMID: 29662258
- Stromal expression of CAV1 in primary tumors was not associated with clinical outcome. However, the stromal expression of CAV2 in metastatic lymph nodes could be associated with lung cancer pathogenesis. PMID: 29850392
- CAV-1 is important for NAFLD-HCC survival in fatty acid-rich environments and is a potential therapeutic target. PMID: 29896915
- At the onset of mitotic cell rounding, caveolin-1 is targeted to the retracting cortical region at the proximal end of retraction fibers. Here, ganglioside GM1-enriched membrane domains with clusters of caveola-like structures are formed in an integrin and RhoA-dependent manner. PMID: 27292265
- Down-regulation of Cav-1 may aggravate DNA damage of Chang liver cells by reducing the interaction of Cav-1 and Mdm2, which promotes p53 degradation. PMID: 29270591
- High CAV1 expression is associated with Small Cell Lung Cancer. PMID: 29479989
- NEDD8 appears to inhibit Src-mediated phosphorylation of caveolin-1 by modifying the structure of caveolin-1 protein, blocking the migration of cancer cells. While the neddylation process is currently considered an emerging target for cancer therapy, these results suggest that inhibition of neddylation could potentially facilitate cancer invasion or metastasis in certain types of cancers. PMID: 29301501
- High CAV1 expression is associated with Aggressive Behavior of Breast Cancer. PMID: 28236153
- Cav1 and PY14Cav1 are positively correlated with ESCC lymphatic metastasis and cancer stages. Rho/ROCK pathway activation promotes ESCC metastasis by regulating Cav1. PMID: 29288243
- Re-examining the relationship between Cav1 and Stat3-ptyr705 in non-transformed mouse fibroblasts and human lung carcinoma cells, this research shows that Cav1 downregulates cadherin-11. This downregulation leads to a reduction in cRac1 and Stat3 activity levels. PMID: 29458077
- Cav-1 acts as a positive or negative regulator of tumor cell growth through the reciprocal control of the RAF-ERK feedback loop. The mitogenic switch of Cav-1 function is tightly linked to bidirectional alteration of its expression in tumor progression. PMID: 29141593
- This is the first demonstration of caveolin-1 expression in human primary uveal melanoma cell lines. The observation that the origin of cells (uveal/cutaneous) impacts the utility of caveolin-I as a melanoma cell marker is noteworthy. PMID: 29847075
- These results suggest that CAV1 protects host cells against Group A Streptococcus invasion by a caveola-independent mechanism. PMID: 28778116
- CAV-1 is commonly downregulated in patients with primary CRC, suggesting its tumor suppressor role in the early stages of this disease. PMID: 28560511
- CAV1 protects Hepatocellular carcinoma cells from TGF-beta-induced apoptosis, attenuating its suppressive effect on clonogenic growth and increasing its effects on cell migration. CAV1 plays a crucial role in switching the response to TGF-beta from cytostatic to tumorigenic, which could have clinical implications for patient stratification. PMID: 29022911
- The finding that Cav1 is both an aggresome-inducing and aggresome-localized protein provides new insights into how cells handle and respond to misfolded Cav1. This raises the possibility that aggresome formation may contribute to some of the reported phenotypes associated with overexpressed and/or mutant forms of Cav1. PMID: 27929047
- High glucose-induced cell senescence in glomerular mesanginal cells is dependent on caveolin-1 signaling. PMID: 27048255
- Caveolin-1 (Cav-1) participates in intraocular pressure maintenance through modulation of aqueous humor drainage from the eye. PMID: 27841369
- This study confirms the association of rs4236601 with primary open-angle glaucoma in different Chinese cohorts. It also found a common single-nucleotide polymorphism rs3801994 with diverse associations with primary open-angle glaucoma between Chinese and Japanese. PMID: 27297022
- Purified caveolin 8S oligomers assumed disc-shaped arrangements of sizes consistent with the discs occupying the faces in the caveolar polyhedra. Polygonal caveolar membrane profiles were revealed in tomograms of native caveolae inside cells. A model with a regular dodecahedron is proposed as the structural basis for the caveolae architecture. PMID: 27834731
- Cav-1 may play a role in the pathogenesis of oral lichen planus and carcinogenesis of squamous cell carcinoma. However, its role in the malignant transformation of OLP is not confirmed. PMID: 28554768
- This research examined the consequences of a familial pulmonary arterial hypertension-associated frameshift mutation in CAV1, P158PfsX22, on caveolae assembly and function. The results show that the P158PfsX22 frameshift introduces a gain of function that gives rise to a dominant negative form of CAV1. This defines a new mechanism by which disease-associated mutations in CAV1 impair caveolae assembly. PMID: 28904206
- This study identified Cav1 and MTCH2 as the molecular targets of DHA and revealed a new link between the upstream Cav1/MTCH2 upregulation and the downstream activation of the cell death pathway involved in DHA-mediated inhibition of cell viability. PMID: 28498397
- Folate deficiency impairs spermatogenesis and reduces sperm concentration, potentially due to the inhibition of the expression of three key molecules (Esr1, Cav1, and Elavl1) essential for sperm production. PMID: 28445960
- Reduced expression of caveolin-1 in monocytes could aggravate the TLR4-mediated inflammatory cascade. PMID: 27981790
- These results suggest that phosphorylated CAV1 activates autophagy by binding to the BECN1/VPS34 complex under oxidative stress, providing protection against ischemic damage. PMID: 28542134
- Caveolin-1 plays a role in promoting Ewing sarcoma metastasis by regulating MMP-9 expression through the MAPK/ERK pathway. PMID: 27487136
- Stromal, but not tumoral, caveolin-1 expression is significantly associated with survival in Asian women with triple-negative breast cancers. PMID: 28735300
- High CAV1 expression is associated with lung cancer. PMID: 26930711
- Kidney transplant patients with high levels of caveolin-1 immunoreactivity in peritubular capillaries (PTCs) had a significantly worse prognosis than patients with lower levels. CAV-1 immunoreactivity in PTCs was independently associated with graft failure. PMID: 27543925
- CAV-1 knockdown by siRNA causes increased radiosensitivity in basal-like TNBC cells. The mechanisms associated with this effect include reduced DNA repair through delayed CAV-1-associated EGFR nuclear accumulation and induction of G2/M arrest and apoptosis through the combined effects of CAV-1 siRNA and radiation. PMID: 29169152
- Fluctuation of reactive oxygen species inhibits migration by reducing the interaction between DLC1 and CAV-1. PMID: 28130753
- This discussion covers current knowledge and future approaches to elucidating the molecular mechanisms underlying CAV1 action during hepatocarcinogenesis and evaluates its potential use in clinical therapies. PMID: 28741517
- Caveolin-1 phosphorylation on tyrosine 14 may play a role in augmenting melanoma metastasis but not tumorigenesis. PMID: 27259249
- These results suggest that Cav-1 may be a predictor of the poor efficacy of EGFR-TKIs treatment in lung adenocarcinoma with EGFR mutations. PMID: 29137977
- This study investigated the effect of Aliskiren on interleukin-6, endothelial nitric oxide synthase, and caveolin-1 in human aortic endothelial cells. Findings suggest that aliskiren reverses the effects of IL-6 on eNOS and caveolin-1 by increasing eNOS phosphorylation and nitric oxide production, decreasing caveolin-1 phosphorylation, and reducing the interaction between eNOS and caveolin-1. PMID: 27773804
- miR-192 is downregulated in rheumatoid arthritis (RA) synovial tissues. Restoring its expression elicits growth-suppressive effects on RA-FLSs by targeting CAV1. The miR-192/CAV1 pathway may represent a novel target for the prevention and treatment of RA. PMID: 28321538
- At the CAV1 gene polymorphism rs926198, minor allele carriers displayed higher odds of insulin resistance and low high-density lipoprotein. Aldosterone levels correlated with higher homeostatic model assessment of insulin resistance and resistin and lower high-density lipoprotein only in minor allele carriers. PMID: 27680666
- These findings highlight the importance of Cav-1 in hematogenous metastasis and provide new insights into the underlying mechanisms of mechanotransduction induced by low shear stress. PMID: 26919102
- Deregulated expression of miR-107 inhibits metastasis of pancreatic ductal adenocarcinoma by inhibiting PI3K/Akt signaling through caveolin-1 and PTEN. PMID: 29111166
Database Links
Involvement In Disease
Congenital generalized lipodystrophy 3 (CGL3); Pulmonary hypertension, primary, 3 (PPH3); Partial lipodystrophy, congenital cataracts, and neurodegeneration syndrome (LCCNS)
Protein Families
Caveolin family
Subcellular Location
Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Membrane raft. Golgi apparatus, trans-Golgi network. Note=Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
Tissue Specificity
Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
