CCDC112 Antibody

Definition and Function of CCDC112 Antibody

CCDC112 antibodies are immunoreagents designed to bind specifically to the CCDC112 protein, a conserved molecular scaffold involved in sperm midpiece formation, centriolar satellite organization, and mitochondrial respiration . These antibodies enable researchers to visualize CCDC112 localization, quantify its expression, and investigate its functional roles using techniques like Western blotting (WB), immunohistochemistry (IHC), and immunocytochemistry (ICC) .

Key Applications of CCDC112 Antibodies

CCDC112 antibodies are utilized in:

• Sperm Biology: Studying mitochondrial sheath assembly and flagellar motility defects in sperm .

• Cancer Research: Investigating cell cycle regulation, particularly delays in G2/M phase transitions .

• Diagnostics: Detecting CCDC112 expression anomalies in male infertility or cancer biopsies .

Role in Male Fertility

CCDC112 is essential for sperm mitochondrial sheath maturation. Knockout studies in mice revealed:

• Structural Defects: Abnormal mitochondrial sheath morphology and reduced ATP production .

• Functional Impairments: Irregular flagellar waveforms and 85% reduction in progressive sperm motility .

• Fertility Impact: Complete male sterility due to failed sperm migration and oocyte penetration .

Cell Cycle Regulation

While CCDC112’s direct role in cancer is less characterized, ectopic overexpression of related coiled-coil proteins (e.g., CCDC110) delays G2/M phase entry in osteosarcoma cells, suggesting a potential regulatory function .

Challenges and Limitations

• Antibody Specificity: Some studies report non-specific binding, complicating localization studies .

• Functional Gaps: The precise molecular mechanisms of CCDC112 in cell cycle control remain unclear .

Future Directions

• Therapeutic Targeting: Explore CCDC112’s potential as a biomarker for male infertility or cancer.

• Mechanistic Studies: Elucidate its interaction with centriolar satellites and mitochondrial proteins .