CCDC8 Antibody

Introduction to CCDC8 Antibody

CCDC8 antibodies are polyclonal or monoclonal immunoglobulins raised against specific epitopes of the CCDC8 protein. They are primarily used in research to investigate CCDC8’s role in:

• HIV-1 inhibition: CCDC8 binds to HIV-1 Gag protein, promoting its endocytosis and lysosomal degradation, thereby blocking viral particle assembly .

• Cancer biology: High CCDC8 expression correlates with poor prognosis in bladder cancer and modulates tumor immune microenvironments .

• Genetic disorders: CCDC8 mutations cause 3-M syndrome, a primordial dwarfism linked to microtubule dysregulation .

Applications in Research and Diagnostics

CCDC8 antibodies are employed in multiple experimental techniques to dissect CCDC8’s cellular and pathological roles:

Western Blotting (WB)

• Purpose: Quantify CCDC8 expression levels in lysates.

• Key Findings:

  • Overexpression of CCDC8 reduces HIV-1 Gag production by ~90% .

  • High CCDC8 expression in bladder cancer correlates with poor survival .

Immunohistochemistry (IHC)

• Purpose: Localize CCDC8 in tissue sections.

• Key Findings:

  • CCDC8 localizes to plasma membranes and cytoplasmic aggregates .

  • In bladder cancer, CCDC8 expression is enriched in basal-type tumors .

Immunofluorescence (IF)

• Purpose: Visualize CCDC8 dynamics in live or fixed cells.

• Key Findings:

  • CCDC8 co-localizes with lysosomal markers (Lamp1-YFP) during Gag internalization .

  • CCDC8-mediated Gag endocytosis occurs at speeds of ~1.53 μm/s .

Immunoprecipitation (IP)

• Purpose: Identify CCDC8 interactome partners.

• Key Findings:

  • CCDC8 interacts with HIV-1 Gag matrix domain and Obsl1/Cul7 complexes .

Research Findings and Functional Insights

CCDC8 antibodies have enabled groundbreaking discoveries in virology and oncology:

HIV-1 Inhibition

• Mechanism: CCDC8 induces Gag polyubiquitination, internalization, and lysosomal degradation, blocking viral egress .

• Therapeutic Potential: Overexpression of CCDC8 could serve as an antiviral strategy, though endogenous CCDC8 levels in CD4+ T cells are low, enabling HIV-1 replication .

Bladder Cancer Prognosis

• Clinical Relevance: High CCDC8 expression predicts poor survival and suboptimal immunotherapy response .

• Immune Modulation: CCDC8 suppresses antitumor immunity by promoting M2 macrophage infiltration and upregulating immune checkpoints (e.g., PD-1, LAG3) .

3-M Syndrome and Microtubule Regulation

• Genetic Role: CCDC8 mutations impair microtubule dynamics and centrosome function, causing primordial dwarfism .

• Interactome: CCDC8 interacts with Obsl1 and Cul7, forming the 3M complex critical for genome integrity .