Exodus 2 Human

Exodus-2 Human Recombinant (CCL21)
Shipped with Ice Packs
In Stock

Description

Biological Function

Exodus-2 binds to the CCR7 receptor, directing immune cell migration and homing. Its roles include:

  • Lymphocyte Homing: Guides T cells, dendritic cells, and B cells to lymphoid organs.

  • Inflammatory Responses: Mediates chronic inflammation by recruiting immune suppressor cells (e.g., Tregs, MDSCs).

  • Disease Pathology: Elevated in rheumatoid arthritis synovial fluid and linked to fibrosis, neuropathic pain, and tumor microenvironments .

Biological Activity:

AssayMethodResultSource
ChemotaxisHuman lymphocyte migration (10–100 ng/mL concentration)Dose-dependent cell migration

Research Applications

Exodus-2 is widely used in immunology and oncology studies:

  • Tumor Microenvironment: Modulates immune cell infiltration and tumor progression.

  • Autoimmune Diseases: Investigated for therapeutic targeting in rheumatoid arthritis and chronic inflammation.

  • Vaccine Development: Explored for enhancing antigen presentation by dendritic cells .

Comparative Analysis of Recombinant Products

Commercially available recombinant Exodus-2 variants share core features but differ in formulation:

VendorFormulationPurityEndotoxinSource
Enzo Life SciencesLyophilized with 0.1% TFA≥95%≤1 EU/µg
ReprokineLyophilized with glycine/sucrose≥95%<0.1 ng/µg
Yeasen BioLyophilized with PB/NaCl buffer>97%<1 EU/µg

Ethical and Regulatory Considerations

  • Research Use Only: Not approved for therapeutic or diagnostic applications.

  • Safety Precautions: Handle with lab coats and gloves to avoid exposure risks .

Product Specs

Introduction
Chemokine (C-C motif) ligand 21 (CCL21), also known as 6Ckine, exodus-2, and secondary lymphoid-tissue chemokine (SLC), is a small cytokine in the CC chemokine family. Unlike other CC chemokines, CCL21 primarily attracts lymphocytes and is mainly found in lymph nodes. The gene responsible for CCL21 is located on human chromosome 9. CCL21 interacts with a cell surface chemokine receptor called CCR7 to exert its effects.
Description
Recombinant Human Exodus-2 is produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 111 amino acids with a molecular weight of 12.2 kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20mM Phosphate Buffer, pH 7.4, and 150mM NaCl.
Solubility
Reconstitute the lyophilized Exodus-2 in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted with other aqueous solutions.
Stability
Lyophilized Exodus-2 is stable at room temperature for up to 3 weeks. However, it is recommended to store the lyophilized product desiccated below -18°C. After reconstitution, CCL21 should be stored at 4°C for 2-7 days. For long-term storage, freeze at -18°C after adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 97.0% as determined by: (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
The biological activity is assessed through the chemoattractant effect on human total lymphocyte population within a concentration range of 10-100 ng/ml.
Synonyms
Small inducible cytokine A21, CCL21, Beta chemokine exodus-2, 6Ckine, Secondary lymphoid-tissue chemokine, SLC, chemokine (C-C motif) ligand 21, ECL, CKb9, TCA4, SCYA21, MGC34555.
Source
Escherichia Coli.
Amino Acid Sequence
SDGGAQDCCL KYSQRKIPAK VVRSYRKQEP SLGCSIPAIL FLPRKRSQAE LCADPKELWV QQLMQHLDKT PSPQKPAQGC RKDRGASKTG KKGKGSKGCR KTERSQTPKG P.

Q&A

What character frequency patterns appear in Exodus 2, and how might researchers quantify these patterns?

Exodus 2 demonstrates a remarkably high density of character references within a brief narrative section. Quantitative textual analysis reveals that of the 25 verses comprising this chapter:

  • 24 verses make specific mention of at least one character

  • 55 total character mentions appear across these verses

  • An average of 2.2 character references occur per verse

  • Approximately 23.6% of all words in the Hebrew text (79 out of 335 total words) directly reference an actor in the narrative

Researchers should employ computational linguistic tools to track pronoun usage, direct naming, and relational descriptors to fully analyze character presence across the text. This frequency analysis provides insight into the narrative's emphasis on human relationships despite the paradoxical anonymity of most characters.

How does the narrative structure of Exodus 2 establish thematic parallels with other biblical liberation narratives?

Methodologically, researchers should approach Exodus 2 as a prologue that establishes the liberation theme central to the broader Exodus narrative. The chapter employs a progressive narrative structure that moves from:

  • Individual oppression (Moses' threatened infancy)

  • Family-level resistance (hiding of Moses)

  • Cross-cultural intervention (Pharaoh's daughter)

  • Interpersonal conflict (Moses intervening in Hebrew conflict)

  • Exile and new community formation (Moses in Midian)

This structural progression establishes a microcosm of the larger liberation narrative and creates literary parallels with other biblical texts where individuals move from marginalization to community formation. Researchers should analyze these patterns using narrative critical methodologies that examine plot development, character functions, and thematic resonance1 .

What methodologies best capture the literary significance of anonymity in Exodus 2?

The pronounced anonymity in Exodus 2 requires specialized literary analysis methods:

  • Contrastive nomination analysis: Compare when names are provided versus when characters are identified only by relationship or function. For example, Moses is the only character named in the early narrative despite numerous other significant figures .

  • Sociopolitical context analysis: Interpret anonymity as a deliberate literary device reflecting the dehumanization of enslaved populations. The text employs anonymity as "a metaphor for the objectification of the Israelites under Egyptian servitude" .

  • Delayed nomination tracking: Document when characters first appear anonymously and when/if they later receive names in the broader narrative.

Advanced researchers should apply these methods while considering the cultural-historical context of name significance in ancient Near Eastern literature.

How should researchers reconcile the numerical discrepancies between biblical accounts of the Exodus population and archaeological evidence?

Methodologically sound approaches to this question require:

  • Critical textual analysis: Examining whether the Hebrew terms in Exodus might represent military units rather than literal headcounts. Some scholars suggest the 600,000 figure might actually represent 600 squads (approximately 3,000-4,000 warriors, suggesting a total population of under 20,000) .

  • Archaeological expectations modeling: Determining what physical evidence would remain from different population sizes:

Proposed Exodus PopulationExpected Archaeological EvidenceCurrent Findings
~1.5 million (traditional)Massive material culture remains, settlement evidenceLimited/inconclusive
~20,000 (reduced interpretation)Modest material remains, possibly overlookedPossible alignment with limited findings
Symbolic narrative onlyNo direct archaeological correlation expectedN/A
  • Comparative ancient demography: Contextualizing claims against known population data - "The Egyptian empire at its population height had three million people in it" .

Researchers should avoid dismissing the narrative entirely while maintaining scholarly rigor in evaluating population claims against archaeological evidence.

What methodological approaches help distinguish historical elements from theological narrative in Exodus 2?

Advanced researchers should employ:

  • Comparative ancient literature analysis: Contextualizing Exodus 2 within similar ancient Near Eastern birth narratives and hero tales to identify conventional literary patterns versus historically plausible elements.

  • Archaeological contextualization: Examining evidence of Jewish populations in Egypt during the proposed time period - "there is some evidence that indicates there was a sizable Jewish population in Egypt roughly around the time that the story of Exodus describes" .

  • Cultural memory theory: Analyzing how historical experiences may have been transformed through generations of oral tradition before textual codification - "The cultural memories of living under Egyptian rule (and possible enslavement) were very real" .

  • Cross-disciplinary verification: Triangulating textual claims with archaeological, anthropological, and historical data to establish degrees of historical probability.

Basic methodology includes simple historical verification, while advanced approaches incorporate nuanced understanding of how ancient texts function as both historical and theological documents.

How does Exodus 2 represent cross-cultural interactions, and what research methodologies best analyze these representations?

Exodus 2 presents multiple cross-cultural interactions requiring sophisticated anthropological analysis:

  • Cultural boundary navigation: Moses moves between Egyptian and Hebrew identities, particularly evident when "he went out to his people and looked on their burdens"1.

  • Power dynamics analysis: Examine the interactions between:

    • Palace authority (Pharaoh's daughter) and marginalized groups (Hebrew midwives)

    • Privileged adopted royal (Moses) and enslaved laborers

    • Egyptian ruling class and Hebrew slaves

    • Male shepherds and female water-drawers in Midian

Researchers should employ ethnographic comparison with known Egyptian-Semitic interactions from historical records while analyzing linguistic markers of cultural difference in the text.

What methodologies help researchers analyze the theological implications of human agency and divine providence in Exodus 2?

Researchers should employ these methodological approaches:

  • Narrative gap analysis: Examine where divine action is explicitly mentioned versus implied. Notably, God is not directly referenced as acting until verse 24, yet the narrative implies divine orchestration of human events.

  • Character motivation analysis: Compare stated versus implied motivations for human actions:

    • Moses' mother's actions (preservation instinct versus faith)

    • Pharaoh's daughter's compassion (humanitarian versus divinely inspired)

    • Moses' intervention in Hebrew conflict (justice seeking versus divine calling preparation)

  • Theological pattern recognition: Identify recurring patterns that suggest divine guidance despite human initiative being foregrounded.

Advanced research approaches examine how this balance between human agency and divine providence establishes theological frameworks that persist throughout liberation narratives1 .

How should researchers methodologically approach the ethical tensions in Moses' violent intervention (Exodus 2:11-15)?

This complex ethical narrative requires multi-faceted analysis:

  • Contextual ethics assessment: Evaluate Moses' actions within ancient Near Eastern ethical frameworks rather than imposing contemporary ethical standards.

  • Narrative critical analysis: Examine how the text itself contextualizes the violence:

    • "He looked this way and that way and seeing no one killed the Egyptian and hid him in the sand"1

    • The text's emphasis on Moses checking if he was seen suggests moral ambiguity

  • Character development tracking: Analyze this episode as a formative ethical development moment in Moses' journey from privileged prince to liberator.

Research methodologies should avoid simplistic moral judgments while examining how this episode functions within the broader narrative of liberation ethics.

What research methods best address the linguistic challenges in translating key terms in Exodus 2?

Advanced linguistic research should employ:

  • Semantic range analysis: Examine Hebrew terms that carry multiple potential meanings:

    • The term translated as "fine" in "she saw that he was a fine baby" (Exodus 2:2) has semantic connections to both physical appearance and divine favor

  • Comparative ancient language assessment: Cross-reference Egyptian and Hebrew naming conventions to better understand terms like "Moses" - "Even Moses may not have had a real name. Contemporary inscriptions indicate that the suffix mose or meses – denoting 'son' or 'child'"

  • Translation variance documentation: Catalog how different translations render key terms and analyze the theological and interpretive implications of these choices.

This methodological approach recognizes that linguistic choices in translation significantly impact theological interpretation.

How do researchers best analyze the significance of naming and anonymity patterns in Exodus 2?

The pronounced anonymity in Exodus 2 requires specialized research approaches:

  • Intentional anonymity analysis: Examine the paradox that this chapter appears in a book titled "Shemot" [Names] yet features almost entirely unnamed characters .

  • Name-giving authority tracking: Document who has the power to name in the narrative (Pharaoh's daughter names Moses) and analyze the socio-political implications.

  • Identity marker cataloging: Document how unnamed characters are identified through:

    • Ethnic markers (Hebrew, Egyptian, Midianite)

    • Familial relationships (daughter, son, wife)

    • Occupational roles (priest, shepherd)

Advanced researchers should connect these naming patterns to broader biblical themes of identity formation and divine recognition of individuals regardless of their social status .

Product Science Overview

Discovery and Structure

Exodus-2 was independently discovered by three research groups from the EST (Expressed Sequence Tag) database . It shares 21-33% identity with other CC chemokines . The protein contains the four conserved cysteines characteristic of β chemokines, plus two additional cysteines in its unusually long carboxyl terminal domain . The theoretical molecular weight of CCL21 is approximately 12.2 kDa .

Expression and Function

CCL21 is expressed in lymph nodes, certain endothelial cells, the spleen, and the appendix . It is known to chemoattract T and B lymphocytes, as well as dendritic cells, to lymph nodes . This chemotactic activity is crucial for the proper functioning of the adaptive immune response. Additionally, CCL21 inhibits hematopoiesis, the process by which blood cells are formed .

Receptors and Signaling

CCL21 signals through the C-C chemokine receptor-7 (CCR7) . This interaction is essential for the migration of immune cells to lymphoid tissues, where they can initiate and regulate immune responses. CCL21 also interacts with the atypical chemokine receptor ACKR4, recruiting beta-arrestin to ACKR4 .

Clinical Relevance

Exodus-2 has been implicated in various clinical conditions. For instance, it plays a role in the migration of lymphocytes and dendritic cells in ulcerative colitis . It may also be involved in the metastasis of pancreatic cancer stem cells . Due to its significant role in immune cell trafficking, CCL21 is a potential target for therapeutic interventions in immune-related diseases and cancer.

Recombinant Production

Human recombinant CCL21 is produced using various expression systems, including E. coli . The recombinant protein is typically lyophilized and can be reconstituted in sterile distilled water or aqueous buffer containing 0.1% BSA . It is used in research to study its biological activity and potential therapeutic applications.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
© Copyright 2024 Thebiotek. All Rights Reserved.